Only a few studies of dental plaque in transmission electron microscopy (TEM) have been performed. The authors have applied TEM for morphometric analysis of dental plaque. Wearing acrylic splints with bovine enamel pieces on the buccal surface of teeth 36 and 46, 21 volunteers rinsed twice a day with a solution of chlorhexidine digluconate, amine/stannous fluoride, or sodium chloride.
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