Effect of biochar origin and soil type on the greenhouse gas emission and the bacterial community structure in N fertilised acidic sandy and alkaline clay soil.

Soil amendment with biochar has received increased attention because of its potential to i) sequester carbon and ii) reduce NO emission when applied to N fertilised soils. To study the effect of biochar origin on greenhouse gas emission in two contrasting soil types, we used a robotized continuous flow incubation system and δC stable isotope approach to compare four biochar types (feed stock: olive mill, corn cob, pistachio shell, cotton stalk) in an alkaline clay soil and two selected biochar types (feed stock: olive mill and corn cob) in an acidic sandy soil. Furthermore, high-throughput sequencing of 16S rRNA genes was performed at the end of the incubation to investigate the effect of different biochars on bacterial community structure in the two different soils.