Enzyme variants in biosynthesis and biological assessment of different molecular weight hyaluronan.

In the present study, low- and high-molecular-weight hyaluronic acids (LMW-HA and HMW-HA) were synthesized in vitro by truncated Streptococcus equisimilis hyaluronan synthases (SeHAS). The enzyme kinetic parameters were determined for each enzyme variant. The MW, structure, dispersity, and biological activity of polymers were determined by electrophoresis, FTIR spectroscopy, carbazole, cell proliferation, and cell migration assay, respectively.

Evaluation of transmembrane domain deletions on hyaluronic acid polymerization of hyaluronan synthase isolated from Streptococcus equisimilis group G.

The membrane enzyme of hyaluronan synthase (HAS) is the key enzyme in hyaluronic acid (HA) biosynthesis by coupling UDP-sugars. Prior studies proposed the C-terminus region of HAS enzyme mediates the production rate and molecular weight of HA. The current study describes the isolation and characterizations of a transmembrane HAS enzyme isolated from Streptococcus equisimilis Group G (GGS-HAS) in vitro.

Introduction of Novel Drug Targets against and Proposing Putative Inhibitors against Adenine N1 (mA22)-tRNA Methyltransferase (TrmK) using Computer-aided Drug Discovery.

Background: Nowadays, the emergence of methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) strains has dramatically restricted the treatment options against this microorganism.

Aim: In this study, we aimed to discover new drug targets and inhibitors against .

Methods: This study consists of two major sections.

Synthesis and characterization of Gd-loaded hyaluronic acid-polydopamine nanoparticles as a dual contrast agent for CT and MRI scans.

Magnetic resonance imaging and computed tomography (CT) suffer from low contrast sensitivity and potential toxicity of contrast agents. To overcome these limitations, we developed and tested a new class of dual contrast agents based on polydopamine nanoparticles (PDA-NPs) that are functionalized and targeted with hyaluronic acid (HA). These nanoparticles (NPs) are chelated with Gd to provide suitable contrast.

Pharmacological effects and therapeutic potential of natural compounds in neuropsychiatric disorders: An update.

Neuropsychiatric diseases are a group of disorders that cause significant morbidity and disability. The symptoms of psychiatric disorders include anxiety, depression, eating disorders, autism spectrum disorders (ASD), attention-deficit/hyperactivity disorder, and conduct disorder. Various medicinal plants are frequently used as therapeutics in traditional medicine in different parts of the world.

Prognostic Value and Biological Role of miR-126 in Breast Cancer.

In eukaryotic organisms such as humans, some noncoding single-stranded RNAs (ncRNAs) contribute to regulating the expression of some genes before and after the transcription process, which in turn controls a number of vital physiological processes, including cell proliferation, differentiation, invasion, angiogenesis, and embryonic development. miR-126 is one of these miRNAs expressed exclusively in endothelial cells such as capillaries and vessels involved in controlling angiogenesis. In recent years, the link between miRs such as miR-126 and the pathology of breast cancer has attracted the attention of many researchers.

Withdrawal Notice: Overview of Enzyme Engineering Towards the Production of Hyaluronic Acid with Tailored Molecular Weight.

Unlabelled: The article has been withdrawn at the request of the author and the editor of the journal Current Protein and Peptide Science. Bentham Science apologizes to the readers of the journal for any inconvenience this may cause. The Bentham Editorial Policy on Article Withdrawal can be found at https://benthamscience.

Transplantation of Stem Cells as a Potential Therapeutic Strategy in Neurodegenerative Disorders.

Stem cells are considered to have significant capacity to differentiate into various cell types in humans and animals. Unlike specialized cells, these cells can proliferate several times to produce millions of cells. Nowadays, pluripotent stem cells are important candidates to provide a renewable source for the replacement of cells in tissues of interest.

Epigenetic modifications in gastric cancer: Focus on DNA methylation.

Gastric cancer is a complex heterogeneous disease which is the fourth prevalent malignancy worldwide. Although, several diagnosis and treatment are available for the gastric cancer patients, however the malignancy is still the third leading cause of cancer-related death in the world. Beside the genetic and environmental factors, epigenetic alterations are also involved in the emergence of gastric cancer.

Comparison of the physicochemical and structural characteristics of enzymatic produced chitin and commercial chitin.

White shrimp (Litopenaeus vannamei) waste is a good source of chitin. However, the chemical chitin extraction generates a large amount of environmental-pollution and increases partial deacetylation of chitin. Therefore, the use of biological extraction is an interesting alternative.

High doses of garlic extract significantly attenuated the ratio of serum LDL to HDL level in rat-fed with hypercholesterolemia diet.

Background: Hypercholesterolemia is associated with an increased risk of heart disease. In this study, we investigated the antihyperlipidemic effects of garlic (Allium sativum L.) in rat models of hypercholesterolemic.

Comparison of the metal concentrations in the feathers of three bird species from southern Iran.

This study was conducted to determine the concentration of metals, namely cadmium, lead, cobalt and copper, in the feathers of chukar (Alectoris chukar), see-see partridge (Ammoperdix griseogularis) and rock dove (Columba livia) in order to: examine the species, gender and age related variations in trace metal accumulation, and identify any relationships between species. Bird samples were collected in February 2012 from the Hormod protected area, southern Iran and the concentration of metals were measured using a Shimadzu AA 660 flame atomic absorption spectrophotometer. The concentrations in all three species were copper > lead > cadmium > cobalt.