Nicotine Increases Macrophage Survival through α7nAChR/NF-κB Pathway in Infection.

Recently, we reported that nicotine plays a role in the failure of the macrophage in the clearance of subspecies (MAP) during infection in Crohn's disease smokers. We also demonstrated that nicotine enhances macrophages cellular survival during MAP infection. Blocking α7 nicotinic acetylcholine receptor (α7nAChR) with the pharmacological antagonist-mecamylamine-subverted the anti-inflammatory effect of nicotine in macrophages.

Development of multiplex PCR and multi-color fluorescent in situ hybridization (-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease.

Background: Several pathogens have been debated to play a role in inflammatory bowel disease (IBD) including Crohn's disease (CD). None of these pathogens have been investigated together in same clinical samples. We developed a multiplex PCR and multi-color fluorescent in situ hybridization (-FISH) protocols for simultaneous detection of CD-associated pathogens including subspecies (MAP), , and adherent-invasive strain LF82.

Genetic Variations of PTPN2 and PTPN22: Role in the Pathogenesis of Type 1 Diabetes and Crohn's Disease.

Genome wide association studies have identified several genes that might be associated with increase susceptibility to Type 1 Diabetes (T1D) and Crohn's disease. Both Crohn's disease and T1D have a profound impact on the lives of patients and it is pivotal to investigate the genetic role in patients acquiring these diseases. Understanding the effect of single nucleotide polymorphisms (SNP's) in key genes in patients suffering from T1D and Crohn's disease is crucial to finding an effective treatment and generating novel therapeutic drugs.