Through bonds or contacts? Mapping protein vibrational energy transfer using non-canonical amino acids.

Vibrational energy transfer (VET) is essential for protein function. It is responsible for efficient energy dissipation in reaction sites, and has been linked to pathways of allosteric communication. While it is understood that VET occurs via backbone as well as via non-covalent contacts, little is known about the competition of these two transport channels, which determines the VET pathways.

Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater.

Allosteric information transfer in proteins has been linked to distinct vibrational energy transfer (VET) pathways in a number of theoretical studies. Experimental evidence for such pathways, however, is sparse because site-selective injection of vibrational energy into a protein, that is, localized heating, is required for their investigation. Here, we solved this problem by the site-specific incorporation of the non-canonical amino acid β-(1-azulenyl)-l-alanine (AzAla) through genetic code expansion.

Note: An automatic liquid nitrogen refilling system for small (detector) Dewar vessels.

Many infrared spectroscopy setups are in principle stable enough to run overnight or longer, but the detector's Dewar vessel must be refilled manually with liquid nitrogen (LN) every couple of hours. Commercial automatic LN refilling systems work reliably only for large Dewars. Here, we present a refilling system which can non-invasively be applied to already installed small Dewars.

Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding.

The impact of the incorporation of a non-natural amino acid (NNAA) on protein structure, dynamics, and ligand binding has not been studied rigorously so far. NNAAs are regularly used to modify proteins post-translationally in vivo and in vitro through click chemistry. Herein, structural characterisation of the impact of the incorporation of azidohomoalanine (AZH) into the model protein domain PDZ3 is examined by means of NMR spectroscopy and X-ray crystallography.

Subhertz spectral fluctuations from high-density excitons in coupled quantum wells.

In 1994 Butov et al. [Phys. Rev.

Absolute negative resistance induced by directional electron-electron scattering in a two-dimensional electron gas.

A three-terminal device formed by two electrostatic barriers crossing an asymmetrically patterned two-dimensional electron gas displays an unusual potential depression at the middle contact, yielding absolute negative resistance. The device displays momentum and current transfer ratios that far exceed unity. The observed reversal of the current or potential in the middle terminal can be interpreted as the analog of Bernoulli's effect in a Fermi liquid.

Probing and controlling the bonds of an artificial molecule.

We demonstrate how molecular quantum states of coupled semiconductor quantum dots are coherently probed and manipulated in transport experiments. The applied method probes quantum states by the virtual cotunneling of two electrons and hence resolves the sequences of molecular states simultaneously. This result is achieved by weakly probing the quantum system through parallel contacts to its constituting quantum dots.

Coherent coupling of two quantum dots embedded in an Aharonov-Bohm interferometer.

We define two laterally gated small quantum dots with less than 15 electrons in an Aharonov-Bohm geometry in which the coupling between the two dots can be changed. We measure Aharonov-Bohm oscillations for weakly coupled quantum dots. In an intermediate coupling regime we study molecular states of the double dot and extract the magnetic field dependence of the coherently coupled states.

Prevalence of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes of Plasmodium falciparum isolates from southern Mauritania.

The increasing resistance of Plasmodium falciparum in the treatment of uncomplicated malaria with pyrimethamine/sulphadoxine has been associated in several studies with the occurrence of point mutations in the genes of dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS). In this study, the prevalence of these mutations was examined in samples from south-east Mauritania, where atypically strong rainfalls in 1998 and 1999 led to a severe outbreak of falciparum malaria. We analysed 386 samples by polymerase chain reaction (PCR) for infection with P.

Modification of the intersubband excitation spectrum in a two-dimensional electron system under a perpendicular magnetic field.

Under an external magnetic field, new branches of spin- and charge-density waves have been studied in a quasi-two-dimensional electron system whose ground state has more than one Landau level occupied by electrons. These collective excitations can be treated as manifestations of the multicomponent nature of the electron system in magnetic field, whereas the occupied Landau levels should be associated with degrees of freedom.

Mesoscopic fast ion conduction in nanometre-scale planar heterostructures.

Ion conduction is of prime importance for solid-state reactions in ionic systems, and for devices such as high-temperature batteries and fuel cells, chemical filters and sensors. Ionic conductivity in solid electrolytes can be improved by dissolving appropriate impurities into the structure or by introducing interfaces that cause the redistribution of ions in the space-charge regions. Heterojunctions in two-phase systems should be particularly efficient at improving ionic conduction, and a qualitatively different conductivity behaviour is expected when interface spacing is comparable to or smaller than the width of the space-charge regions in comparatively large crystals.

Optical investigation of spin-wave excitations in fractional quantum hall states and of interaction between composite fermions.

We demonstrate that the temperature dependence of the electron spin polarization for the fractional states nu = 1/3 and nu = 2/3 displays activated behavior. This study enables the first measurement of the fractional quantum Hall spin-flip gaps. They are found to be systematically larger in comparison with the gaps simultaneously measured in transport.

Enhanced vortex damping by eddy currents in superconductor-semiconductor hybrids.

An enhancement of vortex-motion damping in thin Pb/In superconducting films is obtained through coupling to an adjacent two-dimensional electron gas formed in a modulation-doped GaAs/AlGaAs heterostructure. This effect is observed by monitoring the power dissipation in the superconductor in the vortex state while increasing the density of the electron gas using a gate voltage. Quantitative agreement is found with calculations based on a viscous damping model which considers generation of eddy currents in the electron gas by moving flux lines.

Absence of odd-even parity behavior for Kondo resonances in quantum dots.

Zero-bias anomalies in the conductance through quantum dots have recently been identified as Kondo resonances and explained in terms of the Anderson impurity model. The effect requires a degeneracy and it has been proposed that this should occur for odd electron numbers on the dot. In this paper we present data, obtained on a split-gate quantum dot with a small number of electrons, which are in disagreement with this expectation.

The quantitative characterization of SiGe layers by analysing rocking profiles in CBED patterns.

Convergent beam electron diffraction is used for the quantitative determination of layer thickness, composition and strain in pseudomorphic Si/SiGe two- and three-layer systems grown by molecular beam epitaxy. By using plan-view specimens, we are able to avoid the influence of surface relaxation which generally complicates the determination of strains in cross-sectional specimens. For quantitative strain determination, rocking curves of Bragg lines in energy-filtered convergent beam electron diffraction patterns are analysed.