Comparison of microsuture, interpositional nerve graft, and laser solder weld repair of the rat inferior alveolar nerve.

Purpose: Intraosseous repair of nerves involves difficulty of access and there is concern that bone healing may interfere with repair outcomes. The present report describes the effect of 3 separate repair techniques on recovery from section of the rat intraosseous inferior alveolar nerve, with reference to the mental nerve distal and the trigeminal ganglion proximal to the nerve section.

Materials And Methods: Unilateral exposure of the inferior alveolar nerves of 28 rats was achieved through bone windows.

Simultaneous vascularized bone marrow transplantation to promote acceptance of hind limb allografts and its effects on central and peripheral chimerism.

Administration of donor bone marrow (BM) cells can improve the outcome of transplantation. The ability of donor vascularized bone marrow transplantation (VBM) to provide an ongoing source of donor cells and improve survival in a rigorous rat model of hind limb transplantation (HLTX) was investigated. HLTX were performed between Brown Norway (BN) donors and Lewis recipients in three groups: HLTX; HLTX plus intravenous donor BM cells and HLTX plus simultaneous VBM transplantation.

Microsurgery in common cases of male infertility.

Prior to the advent of microsurgical equipment and techniques, it was rare for urologists or other surgeons to produce a full pregnancy as a result of reconstructing fine tubal blockages in the male reproductive tract. Perhaps 2% of vasectomized men, and a large percent of men born sterile, are desirous of being able to start a pregnancy, and so in 1970, I began a research project with new equipment to find a reliable way of joining fine body tubes. I found that our bodily tubes can be grouped as being three-layered and that their differences lay mainly in the kind of inner, individual mucosal cell structure and their type and thickness of the muscular layer.

How microsurgery can assist in tubal reconstruction.

Despite the fact that gynecologists were very early users of the operating microscope to observe the possible pathology of the cervical mucosa by brilliant lighting and magnification, the advent of in vitro fertilization (IVF) seems to have stopped this specialty from using the benefits of microsurgery in female tubal reconstruction. Now having performed and followed up for at least 2 years nearly 1000 microsurgical tubal reconstructions after sterilizations, with an endpoint being the birth of a baby in the time following, it is obvious that there are real benefits in pursuing these techniques, because no single IVF Unit in the world gets even half as many live birth results from their best practices. Apart from a genuine 82.

Pathological score for the evaluation of allograft rejection in human hand (composite tissue) allotransplantation.

The experience obtained from the human hand allografts (HHA) performed to date suggests that the skin is a priviledged target of allograft rejection in this setting. The aim of this study was to establish a pathological score for assessing the severity of HHA rejection. The pathological slides of 89 skin biopsies obtained from the allografted limbs of six HHA recipients from day 0 to 5 years post-graft were retrospectively examined.

Clinicopathologic features of graft rejection of the first human hand allograft.

Background: The first human hand allograft, performed in Lyon, France, on September 23, 1998, was removed during month 29 posttransplantation as the result of rejection because the patient did not comply with the immunosuppressive treatment.

Methods: The patient was regularly examined from the day of transplantation to amputation. Biopsies were taken from the skin of the allograft and examined immunohistologically.

Functional results of the first human double-hand transplantation.

Objective: Objective of this study was to analyze fifteen months after surgery the sensorimotor recovery of the first human double hand transplantation.

Summary Background Data: As for any organ transplantation the success of composite tissue allografts such as a double hand allograft depends on prevention of rejection and its functional recovery.

Methods: The recipient was a 33-year-old man with bilateral amputation.

Long-term result of guided nerve regeneration with an inert microporous polytetrafluoroethylene conduit.

Objective: To evaluate the long-term outcome of Polytetrafluoroethylene (PTFE) conduit in nerve repair and to provide more evidence in view of its potential application to achieve a satisfactory functional recovery in clinical settings.

Methods: Thirty-six Wistar rats had their right sciatic nerve transected and were repaired with either conventional microsuture technique (Control group, n=18) or a PTFE conduit with a gap of 5 mm left between the nerve stumps (PTFE group, n=18). At 6 and 9 months after the operation, electrophysiological assessment and measurement of gastrocnemius muscle weight were conducted and morphology of the regenerated nerves were studied with image analysis.

Effect of FK506 and basic fibroblast growth factor on nerve regeneration using a polytetrafluoroethylene chamber for nerve repair.

Peripheral nerve repair can be accomplished by using a polytetrafluoroethylene tubular chamber to guide nerve healing and regeneration. In this study, we delivered basic fibroblast growth factor (bFGF) into the chamber for sciatic nerve repair in rats. In addition, the animals were given systemically 1 mg/kg/day FK506 (tacrolimus), a potent immunosuppressant with neurotrophic properties.

Low dose discontinued FK506 treatment enhances peripheral nerve regeneration.

FK506 has been reported to enhance nerve regeneration in various rodent models. However, both the immunosuppressive properties and potential side effects may preclude the broad clinical use of FK506 to speed nerve regeneration. In this study, we examined the nerve regenerative property of a low, nonimmunosuppressive dose (0.