Cyclic electrographic seizures in critically ill patients.

Purpose: Prolonged electroencephalographic monitoring has facilitated the detection of nonconvulsive seizures. Compressed displays of EEG frequency spectra (such as compressed spectral array, CSA) can facilitate interpretation of continuous EEG by allowing the reader to observe on a single screen patterns evolving over many minutes or hours.

Methods: Patients were identified retrospectively over a 4-year period as displaying a cycling pattern of seizures on CSA.

Electrographic seizures and periodic discharges after intracerebral hemorrhage.

Objective: To determine the frequency and significance of electrographic seizures and other EEG findings in patients with intracerebral hemorrhage (ICH).

Methods: We reviewed 102 consecutive patients with ICH who underwent continuous electroencephalographic monitoring (cEEG). Demographic, clinical, radiographic, and cEEG findings were recorded.

Molecular genetic evidence of an independent origin of serous low malignant potential implants and lymph node inclusions.

Patients with ovarian serous tumors of low malignant potential (LMP) are commonly found to have peritoneal implants. Less commonly, similar lesions are seen in lymph nodes, sometimes in association with endosalpingiosis. We compared these lesions to the coexisting ovarian LMP tumors to determine whether they are clonally related to the ovarian neoplasm.

Molecular genetic evidence supporting the neoplastic nature of the Leydig cell component of ovarian Sertoli-Leydig cell tumors.

Sertoli-Leydig cell tumors (SLCT) comprise less than 1% of ovarian tumors. The nature of the Leydig cells has been a subject of controversy and it is unclear whether they are clonally related to the neoplasm or instead proliferate as a non-neoplastic response to the Sertoli-cell component. Twelve ovarian SLCT were identified and hematoxylin and eosin and unstained sections were prepared from formalin-fixed, paraffin-embedded tissue blocks.

Enhanced peritoneal ovarian tumor dissemination by tissue transglutaminase.

Tissue transglutaminase (TG2) is involved in Ca(2+)-dependent aggregation and polymerization of proteins. We previously reported that TG2 mRNA is up-regulated in epithelial ovarian cancer (EOC) cells compared with normal ovarian epithelium. Here, we show overexpression of the TG2 protein in ovarian cancer cells and tumors and its secretion in ascites fluid and define its role in EOC.

Preoperative and postoperative limb alignment after Oxford unicompartmental knee arthroplasty.

From a series of 64 sequential Oxford (Biomet Orthopedics, Inc, Warsaw, phase 3 implants, two study groups were selected: 23 knees with unilateral surgery and a normal opposite side, and 21 knees with bilateral simultaneous surgery. Standing 3-foot radiographs were taken pre- and postoperatively to assess the femorotibial angle and the location of the mechanical axis of the limb in relation to the knee. Results in the unilateral group showed an average postoperative femorotibial angle of 4.

Design and testing of classroom and clinical teaching evaluation tools for nursing education.

Student evaluations of teaching provide administrators an overall picture of the effectiveness of personnel and contribute data for promotion and merit decisions. These evaluations must be assessed for their relevance, validity, and reliability. This paper describes the development process and psychometric testing for clinical (n = 149) and didactic (n = 148) student evaluation of teaching forms for undergraduate and graduate courses in one college of nursing.

Morphological approach to tumours of the testis and paratestis.

Most neoplastic scrotal masses ultimately prove to be germ cell tumours and are recognisable with routine haematoxylin and eosin-stained sections. The differential diagnosis may be focused, even before reviewing histological sections, by knowledge of patient age, medical history, tumour site (testicular vs paratesticular) and gross findings. Some cases may prove to be diagnostically challenging, including rare tumours, a common tumour with an unusual pattern, a metastatic tumour, or a neoplasm with features that mimic another tumour.

Benign glandular inclusions a rare cause of a false positive sentinel node.

An 84-year-old female underwent a wire-guided lumpectomy and sentinel lymph node biopsy. Two sentinel nodes were identified and sent for immediate pathological evaluation. One of the nodes was reported as "glandular epithelium consistent with metastatic adenocarcinoma.

Cortical abnormalities in epilepsy revealed by local EEG synchrony.

Abnormally strong functional linkage between cortical areas has been postulated to play a role in the pathogenesis of partial epilepsy. We explore the possibility that such linkages may be manifest in the interictal EEG apart from epileptiform disturbances or visually evident focal abnormalities. We analyzed samples of interictal intracranial EEG (ICEEG) recorded from subdural grids in nine patients with medically intractable partial epilepsy, measuring interelectrode synchrony using the mean phase coherence algorithm.

Frequency and predictors of nonconvulsive seizures during continuous electroencephalographic monitoring in critically ill children.

Objective: To determine the incidence, predictors, and timing of nonconvulsive seizures (NCSz) during continuous electroencephalographic monitoring (cEEG) in critically ill children.

Methods: We identified critically ill children who underwent cEEG during a 4-year period. Multivariate logistic regression analysis was performed to determine variables associated with NCSz.

Microscale correlation between surface chemistry, texture, and the adhesive strength of Staphylococcus epidermidis.

Staphylococcus epidermidis is among the most commonly isolated microbes from medical implant infections, particularly in the colonization of blood-contacting devices. We explored the relationships between surface wettability and root-mean-square roughness (Rq) on microbial adhesive strength to a substrate. Molecular-level interactions between S.

The megakaryocyte lineage originates from hemangioblast precursors and is an integral component both of primitive and of definitive hematopoiesis.

In the adult, platelets are derived from unipotential megakaryocyte colony-forming cells (Meg-CFCs) that arise from bipotential megakaryocyte/erythroid progenitors (MEPs). To better define the developmental origin of the megakaryocyte lineage, several aspects of megakaryopoiesis, including progenitors, maturing megakaryocytes, and circulating platelets, were examined in the murine embryo. We found that a majority of hemangioblast precursors during early gastrulation contains megakaryocyte potential.

Magnetoencephalography in epilepsy: tailoring interpretation and making inferences.

Although the ability of magnetoencephalography (MEG) to detect epileptiform discharges noninvasively has long been known, only recently has it become a common tool in clinical settings. Whether MEG or electroencephalography (EEG) is superior has been controversial; MEG has a theoretic edge over EEG for precise localization, but EEG has many practical advantages. Experience has shown that they often provide different and complementary information.

Endoscopic ultrasound-guided fine-needle aspiration cytology diagnosis of intraductal papillary mucinous neoplasm of the pancreas is highly predictive of pancreatic neoplasia.

Intraductal papillary mucinous neoplasms (IPMN) have been considered difficult to diagnose by fine-needle aspiration (FNA) cytology. We identified 57 cases diagnosed as IPMN or consistent with IPMN by endoscopic ultrasound (EUS)-guided FNA over a 9-yr period. Histologic follow-up was available for 20 patients (35%).

Prognostic significance of continuous EEG monitoring in patients with poor-grade subarachnoid hemorrhage.

Introduction: Predicting outcome in patients with poor-grade subarachnoid hemorrhage (SAH) may help guide therapy and assist in family discussions. The objective of this study was to determine if continuous electroencephalogram (cEEG) monitoring results are predictive of 3-month outcome in critically ill patients with SAH.

Methods: We prospectively studied 756 patients with SAH over a 7-year period.

Chaperone-like activity revealed in the matricellular protein SPARC.

SPARC (Secreted Protein, Acidic and Rich in Cysteine) is a matricellular glycoprotein that modulates cell proliferation, adhesion, migration, and extracellular matrix (ECM) production. In this report chaperone-like activity of SPARC was identified in a thermal aggregation assay in vitro. Ultraviolet circular dichroism (UVCD) spectroscopy determined that SPARC was stable at temperatures up to 50 degrees C.

Chromosome 12p abnormalities in dysgerminoma of the ovary: a FISH analysis.

Dysgerminoma is the most common malignant ovarian germ cell tumor and shares histological and immunophenotypical features with its testicular counterpart, seminoma. Chromosome 12p abnormalities are genetic hallmarks of testicular seminomas. Little is known about these genetic changes in dysgerminoma.

The use of immunohistochemistry in the differential diagnosis of tumors of the testis and paratestis.

Although most testicular and paratesticular tumors can be recognized by their light microscopic features, some raise significant differential diagnostic questions. Immunohistochemical staining has proved of significant value in this situation. There is still a role for the traditional markers, including placental-like alkaline phosphatase and alpha-fetoprotein, but newer markers provide additional support and often have greater sensitivity and specificity for many diagnoses.

Immunohistochemical markers in the evaluation of tumors of the urinary bladder: a review.

The clinical utility of immunohistochemistry in the diagnosis of tumors of the urinary bladder is well established. With recent advances in molecular biology and novel technologies, several biomarkers have emerged as important adjuncts in the diagnosis of lesions of the bladder. When used in conjunction with careful histologic examination, immunohistochemistry can be a valuable aid in classifying adenocarcinoma presenting in the bladder and mesenchymal lesions of the bladder and in establishing the urothelial origin of a metastatic tumor.

On the importance of precise calibration techniques for an atomic force microscope.

Proper calibration of any instrument is vital to an investigator's ability to compare laboratory experiments, as well as to draw quantitative relations between experimental results and the real world. For the atomic force microscope, knowledge of quantities such as the probe spring constant, the piezoactuator voltage/height response, and the probe radius of curvature is necessary when transforming raw data into height, separation and force. These parameters are also prerequisites when applying mathematical models to the collected data.