Sarcoplasmic calcium-binding proteins in protochordate and cyclostome muscle: characterization of a new protein from amphioxus.

A sarcoplasmic calcium-binding protein (SCP) has been purified from the muscle of the protochordate Amphioxus and shown to be more similar to invertebrate SCP's than to their counterpart found in vertebrates, i.e. parvalbumins.

Electron-microscopic observations of normal coelomocytes from the earthworm, Lumbricus terrestris.

Coelomocytes of the earthworm, Lumbricus terrestris, were studied by transmission electron microscopy. Four morphological cell types are distinguishable: lymphocytic coelomocytes, granulocytic coelomocytes, eleocytes (chloragogen cells), and inclusion-containing coelomocytes. Within these major categories, several distinct cell types differ and may represent developmental stages.

Graft rejection in earthworms: an electron microscopic study.

Graft rejection in the earthworm, Lumbricus terrestris, is mediated by granulocytic coelomocytes (neutrophils, by light microscopy). Within 1--3 days post-transplantation of body-wall xenografts, granulocytic coelomocytes migrate to the transplant site, penetrate the graft matrix, and actively phagocytize viable muscle fibers. Destruction of muscle tissues may be augmented by lymphocytic coelomocytes (basophils, by light microscopy), but these cells participate in a minor way and do so very late in the rejection process (days 11--13).

Familial hypo-beta-lipoproteinemia: a family detected by cord blood tests.

A family with low-density lipoprotein (LDL) deficiency was detected during the course of screening cord blood samples. The initial diagnosis in the proband was based on the cord blood LDL cholesterol and lipoprotein electrophoretic pattern, and was confirmed by repeated studies at the age of 8 months. The infant had none of the clinical abnormalities previously ascribed to the condition.

The lipid disorders centre at the Transvaal Memorial Hospital for Children. A review of the first 30 months.

A specialized clinic for the investigation and treatment of familial lipoprotein disorders was started at the Transvaal Memorial Hospital for Children in July 1972. The reasons for its formation, its siting at a paediatric hospital and patient protocols are detailed. During the first 30 months, 195 patients with familial hyperlipoproteinaemia were seen.

Comparative properties of vertebrate parvalbumins.

Pure parvalbumins isolated from turtle, chicken, and rabbit white skeletal muscle have been characterized in terms of their physical, chemical, and immunological properties. As for the parvalbumins of most fish and amphibians, they have sedimentation constants S20,w of approximately 1.45 +/- 0.

Morphine effects on the cardiovascular system of awake, freely behaving rats.

The effects of small, i.v. infusion of morphine on the heart rate was studied in unanesthetized rats.

An analysis of 70 years of morphine classical conditioning: implications for clinical treatment of narcotic addition.

A review of all morphine classical conditioning studies performed since Pavlov reveals significant shifts in methodological and conceptual thinking during the past 7 decades. These shifts have tended to obscure the fact that a great deal of basic data are still needed before morphine classical conditioning can be understood. These data are needed to help clarify the relationship between drug conditioning and drug addiction.

Isolation and properties of a sarcoplasmic calcium-binding protein from crayfish.

The sarcoplasmic calcium-binding protein from crayfish muscle has been purified to homogeneity. The protein has a molecular weight of 44000, as determined by sedimentation equilibrium and Sephadex chromatography. It dissociates in the presence of sodium dodecyl sulfate, 8 M urea, or, after succinylation, into two subunits of 22000 molecular weight.

On the subunit structure of particulate aminopeptidase from pig kidney.

Solubilization of particulate aminopeptidase (EC 3.4.11.

Lowering of plasma cholesterol levels in free-living adolescent males; use of natural and synthetic polyunsaturated foods to provide balanced fat diets.

Two hundred and twenty-nine adolescent male pupils, attending two boarding schools, participated in a study, under free-living dietary conditions, designed to assess the effects on plasma lipids of altering only the type and not the amount of dietary fat. The students were monitored for 6 weeks on three different diets. During the first study period, dietary changes comprised substituting a polyunsaturated dried "filled" milk and products derived therefrom for conventional dairy products (diet A).

Colestipol, clofibrate, cholestyramine and combination therapy in the treatment of familial hyperbetalipoproteinaemia.

Fifty-seven patients, mean age 26 years, suffering from familial hyperbetalipoproteinaemia (Fredrickson type lla and llb), were treated on a low cholesterol, modified polyunsaturated fat diet for a period of 6-12 weeks prior to the introduction of drug therapy. No significant reduction in the serum levels of total cholesterol, low density lipoprotein (LDL) cholesterol or triglyceride was found. Fifty patients were then treated with colestipol for 6 weeks; total and LDL cholesterol decreased by 23%, but triglyceride levels were unaffected.

Portacaval shunt in four patients with homozygous hypercholesterolaemia.

Four patients with homozygous hyperbetalipoproteinaemia who had proved resistant to intensive medical therapy have undergone portacaval shunt. During 3 weeks of total parenteral alimentation before the operation, the serum-cholesterol decreased significantly in each of the four patients. During the fat-emulsion phase of hyperalimentation, one patient experienced a rise in cholesterol.