Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes.

A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.

[Identification of Target Extracellular Proteases--Activators of Proteins of Haemostasis System Produced by Micromycetes Aspergillus ochraceus and Aspergillus terreus].

Effects of extracellular proteases of Aspergillus ochraceus and Aspergillus terreus on plasma hemostasis proteins, consist of initiating the activation of prothrombin complex proteins, was detected. Was discovered, that A. ochraceus proteases have a direct influence on protein C and coagulation factor X, and A.

[Effect of extracellular proteases of micromycetes of the genus Aspergillus on proteins of haemostasis system].

Screening of the genus Aspergillus micromycetes for secretion of extracellular proteolytic enzymes, capable of acting on human proteins of the hemostatic system, has been conducted. The ability of extracellular proteases of Aspergillus to cleave specific proteins of the hemostatic system chromogenic peptide substrates, as well as activate a series of proenzymes (protein C, factor X and prothrombin) has been found. The ability of extracellular proteases of micromycetes activate X factor of human blood plasma was first shown at the first time.

Amputee Hockey: Biomechanical Evaluations, Problems, and Paralympic Outlook.

The first ever ice hockey team of transtibial amputees playing in a standing position was established in St. Petersburg in 1999 under the program "US-Russian Prosthetic Rehabilitation Bridge" [1]. Nowadays, Canada, Russia, USA, Finland, Czech Republic and Latvia have their national associations, two World Championships were conducted (2003 - Kiisakalio, Finland; 2004 - Prague, Czech Republic), and the International Standing Ice Hockey Federation (ISIHF) was formed.