Determination of a new hypoxia selective agent from 2-quinoxalinecarbonitrile 1,4-di-N-oxides in plasma by high performance liquid chromatography.

A high performance liquid chromatography (HPLC) method was developed and validated for the determination of 7-chloro-3-[[(N,N-dimethylamino)propyl]amino]-2-quinaxolinecarbonitri le 1,4-di-N-oxide (Q-85), a new hypoxia-selective agent, in plasma. The assay involves extraction into diethylether-dichloromethane (2:1) from plasma and subsequent analysis by reversed-phase HPLC with ultraviolet detection at 292 nm. Response was linear in the range of 0.

Physico-chemical properties and stability of the new hypoxia-selective agent 7-chloro-3-[[N,N-dimethylamino)propyl]amino]-2-quinoxalinecarbonitrile 1,4-di-N-oxide hydrochloride.

7-Chloro-3-[[N,N-dimethylamino)propyl]amino]-2-quinoxalinecarbonitril e 1,4-di-N-oxide hydrochloride (Q-85 HCl), a new hypoxia-selective agent, was studied to clarify its physico-chemical properties. Melting point, spectra (UV, IR, NMR, MS), thermal behaviour, solubility, pKa, partition coefficient and chromatographic characteristics (TLC, HPLC) have been determined. The stability of Q-85 HCl was studied under various conditions by HPLC.