Publications by authors named "E Wiesner"
Recent clinical trials of drugs enhancing cyclic guanosine monophosphate (cGMP) signaling for cardiovascular diseases have renewed interest in cGMP biology within the kidney. However, the role of cGMP signaling in glomerular endothelial cells (GECs) and podocytes remains largely unexplored. Using acute kidney slices from mice expressing the FRET-based cGMP biosensor cGi500 in endothelial cells or podocytes enabled real-time visualization of cGMP.
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- - Formaldehyde (FA) is a harmful toxin linked to cancer and aging, with genetic mutations in detoxifying enzymes leading to its accumulation and severe health consequences in conditions like AMeD syndrome.
- - The study examined how various DNA repair systems in *C. elegans* respond to excessive FA, identifying three distinct repair mechanisms involved in managing FA-induced DNA damage throughout development and reproduction.
- - Results indicate that the Cockayne syndrome B (CSB) factor plays a key role in resolving FA-related DNA damage, and the antioxidant N-acetyl-l-cysteine (NAC) could offer a therapeutic approach to mitigate FA's harmful effects.
In recent years functional multiphoton (MP) imaging of vital mouse tissues and stimulation emission depletion (STED) imaging of optically cleared tissues allowed new insights into kidney biology. Here, we present a novel workflow where MP imaging of calcium signals can be combined with super-resolved STED imaging for morphological analysis of the slit diaphragm (SD) within the same glomerulus. Mice expressing the calcium indicator GCaMP3 in podocytes served as healthy controls or were challenged with two different doses of nephrotoxic serum (NTS).
View Article and Find Full Text PDFMycophenolate mofetil (MMF) is applied in proteinuric kidney diseases, but the exact mechanism of its effect on podocytes is still unknown. Our previous in vitro experiments suggested that MMF can ameliorate podocyte damage via restoration of the Ca-actin cytoskeleton axis. The goal of this study was to characterize podocyte biology during MMF treatment in nephrotoxic serum (NTS) nephritis (NTN).
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