High-performance liquid chromatography of sulphadimethoxine and its N1-glucuronide, N4-acetyl and N4-acetyl-N1-glucuronide metabolites in human plasma and urine.

Sulphadimethoxine is metabolized in humans by N1-glucuronidation and by N4-acetylation. Sulphadimethoxine-N1-glucuronide can be measured by the direct high-performance liquid chromatographic analysis and without enzymic deglucuronidation. The N1-glucuronide can be measured by an isocratic as well as by a gradient mobile phase.

N-oxidation, O-demethylation, and excretion of trimethoprim by the turtle Pseudemys scripta elegans.

Trimethoprim was excreted within one hour after being given orally to the turtle Pseudemys scripta elegans. No O-demethylated or N-oxide metabolites were detected.