[Dual role of photosensibilizator merocyanine 540 as a bactericidal agent and immune reaction regulator].

Aim: Develop conditions for inactivation of staphylococcus by using photosensibilizator merocyanine 540 (MC540) for the production of antigenic preparation (AP). Study some of immune reactions to AP and the possibility of regulation of DTH reaction to AP under the effect of MC540.

Materials And Methods: Merocyanine 540 (MC540, Sigma-Aldrich, Switzerland) is used in the study.

[Bactericidal action of non-thermal plasma on biofilms formed in vitro and within a root channel].

Aim: Evaluation of efficiency of non-thermal plasma as bactericidal agent affecting biofilms formed in vitro and on walls of a root channel.

Materials And Methods: The multiple antibiotic resistant strain Staphylococcus epidermidis isolated from pulpitis was used. Biofilms formed in vitro on the plastic surface and ex vivo at the walls of the root canal were treated with plasma torch formed by argon:air (9:1) mixture eradiated with 100 kHz electrtomagnetic field.

[New approaches to therapy of persistent infections: elimination of intracellular Chlamydai trachomatis by exposure to low temperature argon plasma].

Aim: Study microbicidal activity of low temperature argon plasma (LTP) that is a stream of partially ionized argon having macroscopic temperature of the environment against Chlamydia trachomatis obligate intracellular parasites. Study viability of host cells in parallel.

Materials And Methods: McCoy line cells infected with C.

[Prospects for the use of low-temperature gas plasma as an antimicrobial agent].

Results of application of LTP at atmospheric pressure as an antibacterial agent during the last decade are considered with reference to physicochemical mechanisms of its bactericidal action. The principles of designing modern LTP sources are described in conjunction with the results of LTP application against pathogenic bacteria in vitro and in biofilms. The possibility to destroy biofilm matrix by LTP is estimated along with the results of its testing for the treatment of acute and chronic wound surfaces.

[Immunobiological features of bacterial cells of medical biofilms].

Biofilm is a integrated multucellular organism with own cycle of development, cooperative behavior of units forming it, which coordinated by QS-system based on production of signal molecules or autoinductors and ability of bacteria to receive these signals. Presence ofbacteria attached to surface of biomedical devices and formation of bacterial biofilms in the microorganism could lead to chronic inflammation, which characterized by presence of macrophages and lymphocytes in the focus of inflammation as well as proliferation of connective tissue, accumulation of matrix proteins and stimulation ofangiogenesis. Process of biofilm formation associated with activation of QS-system of different agents including potentially dangerous bacteria plays certain role in exacerbation of gastric and duodenal ulcer diseases, Crohn's disease, myocarditis, asthma, diabetes mellitus, cardiovascular and other somatic diseases.