Deletion of increases luminescence of .

Unlabelled: Bioluminescence in is regulated by a quorum-dependent signaling system composed of LuxI and LuxR. LuxI generates 3-oxohexanoyl homoserine lactone (3OC6-HSL), which triggers LuxR to activate transcription of the operon responsible for bioluminescence. Surprisingly, a ∆ mutant produced more bioluminescence than the wild type in culture.

Transient infection of with an engineered D-alanine auxotroph of .

The symbiosis between and the Hawaiian bobtail squid, , is a tractable and well-studied model of bacteria-animal mutualism. Here, we developed a method to transiently colonize using D-alanine (D-ala) auxotrophy of the symbiont, controlling the persistence of viable infection by supplying or withholding D-ala. We generated alanine racemase () mutants of that lack avenues for mutational suppression of auxotrophy or reversion to prototrophy.

Massively parallel mutant selection identifies genetic determinants of colonization of .

is recognized for its ability to colonize diverse habitats and cause disease in a variety of hosts, including plants, invertebrates, and mammals. Understanding how this bacterium is able to occupy wide-ranging niches is important for deciphering its ecology. We used transposon sequencing [Tn-Seq, also known as insertion sequencing (INSeq)] to identify genes in that contribute to fitness during the colonization of .

Massively parallel mutant selection identifies genetic determinants of colonization of .

is recognized for its ability to colonize diverse habitats and cause disease in a variety of hosts, including plants, invertebrates, and mammals. Understanding how this bacterium is able to occupy wide-ranging niches is important for deciphering its ecology. We used transposon sequencing (Tn-Seq, also known as INSeq) to identify genes in that contribute to fitness during colonization of .