MS Identification of Blood Plasma Proteins Concentrated on a Photocrosslinker-Modified Surface.

This work demonstrates the use of a modified mica to concentrate proteins, which is required for proteomic profiling of blood plasma by mass spectrometry (MS). The surface of mica substrates, which are routinely used in atomic force microscopy (AFM), was modified with a photocrosslinker to allow "irreversible" binding of proteins via covalent bond formation. This modified substrate was called the AFM chip.

Synergistic Interaction of 5-HT and 5-HT Receptors in Cytoplasmic Ca Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies.

The aim of this work was to explore the involvement of 5-HT and 5-HT receptors (5-HTR and 5-HTR) in the regulation of free cytoplasmic calcium concentration ([Ca]) in human umbilical vein endothelial cells (HUVEC). We have shown by quantitative PCR analysis, that 5-HTR and 5-HTR mRNAs levels are almost equal in HUVEC. Immunofluorescent staining demonstrated, that 5-HTR and 5-HTR are expressed both in plasma membrane and inside the cells.

Child self-care autonomy in health (scale for parents): development, internal structure, and sex/age correlates.

Autonomy in self-care practices in the health sphere is a critical characteristic for the survival of humans throughout the life span. Notably, however, the current literature lacks psychometrically sound instruments that measure this phenomenon among children without diagnosed chronic health conditions. The purpose of the present exploratory study was to develop, test, and provide data regarding the reliability and validity of the Child Self-Care Autonomy in Health (CSAH) scale.

Mass Spectrometric Identification of BSA Covalently Captured onto a Chip for Atomic Force Microscopy.

Mass spectrometry (MS) is one of the main techniques for protein identification. Herein, MS has been employed for the identification of bovine serum albumin (BSA), which was covalently immobilized on the surface of a mica chip intended for investigation by atomic force microscopy (AFM). For the immobilization, two different types of crosslinkers have been used: 4-benzoylbenzoic acid N-succinimidyl ester (SuccBB) and dithiobis(succinimidyl propionate) (DSP).

SARS-CoV-2 Receptors and Their Involvement in Cell Infection.

The new coronavirus infection (COVID-19) pandemic caused by SARS-CoV-2 has many times surpassed the epidemics caused by SARS-CoV and MERS-CoV. The reason for this was the presence of sites in the protein sequence of SARS-CoV-2 that provide interaction with a broader range of receptor proteins on the host cell surface. In this review, we consider both already known receptors common to SARS-CoV and SARS-CoV-2 and new receptors specific to SARS-CoV-2.