[Role of DNA repair genes in radiation-induced changes of lifespan of Drosophila melanogaster].

One of the main effects of various stress factors, including ionizing radiation, is DNA damage. Accumulation of DNA damage and somatic mutations in the somatic tissues is regarded as one of the basic mechanisms of aging. We have developed an approach to the study of molecular and genetic mechanisms of radioadaptation, which is based on the analysis of changes in the lifespan of Drosophila with a transformed genotype.

[Recessive lethal mutation in X-cromosome locus 6D1-7A2 in heterozygote leads to life span increasing in Drosophila melanogaster].

The results of demographic analysis of survival of different Drosophila lines have shown that recessive lethal mutation located in X-chromosome locus 6D1-7A2, as compared with wild type strain, in heterozygote leads to aging speed deceleration (decreasing of mortality trajectory slope), median (20%) and maximal (15-20%) life span increasing. Further investigations (complementation analysis with duplicated loci) will enable to discover, whether it is unknown life span gene.

[The influence of low doze gamma-irradiation on life span of Drosophila mutants with defects of DNA damage sensation and repair].

It was shown that life span of wild type strain Canton-S increased after low doze gamma-irradiation. It was revealed the decrease of life span after irradiation in Drosophila mutants with defects of DNA damage sensation and repair genes mei-9 and mei-41, both in homozygous and heterozygous lines. In mei-41 line males' lives longer than females in contrast to other lines.

[A new approach to the analysis of complex chromosomal rearrangements in cell hybrids].

The chromosomal complements of somatic cell pig-mink hybrids was determined by a new approach. This approach includes microdissection of metaphase chromosomes, generation of chromosome and region-specific DNA libraries, and fluorescence in situ hybridization of these libraries with pig lymphocyte chromosomes. The studied hybrid cells were shown to contain two small acrocentric chromosomes and a microchromosome of porcine origin.