C-Terminal Region of Caveolin-3 Contains a Stretch of Amino Acid Residues Capable of Diminishing Symptoms of Experimental Autoimmune Encephalomyelitis but Not Rheumatoid Arthritis Modeled in Rats.

A short synthetic peptide from the C-terminal part of the caveolin-3 structure was tested for experimental autoimmune encephalomyelitis (EAE) treatment in rats. The structure-function similarity established between the novel synthetic peptide of pCav3 and the well-known immunomodulator immunocortin determined pCav3's ability to reduce EAE symptoms in Dark Agouti (DA) rats injected with pCav3 (500 µg/kg). pCav3 was found to interfere with the proliferation of lymphocytes extracted from the LNs of DA rats primed with homogenate injection, with IC = 0.

Binding of cholera toxin B subunit to intestinal epithelial cells.

We have prepared I-labeled cholera toxin B subunit (I-labeled CT-B, a specific activity of 98Ci/mmol) and found that it binds to rat IEC-6 and human Caco-2 intestinal epithelial cells with high affinity (K 3.6 and 3.7nM, respectively).

Interaction of Cholera Toxin B-subunit with Human T-lymphocytes.

In this work, I-labeled cholera toxin B-subunit (CT-B) (specific activity 98 Ci/mmol) was prepared, and its high-affinity binding to human blood T-lymphocytes (K = 3.3 nM) was determined. The binding of the I-labeled CT-B was inhibited by unlabeled interferon-α (IFN-α), thymosin-α (TM-α), and by the synthetic peptide LKEKK, which corresponds to sequences 16-20 of human TM-α and 131-135 of IFN-α (K 0.

Interaction of cholera toxin B subunit with T and B lymphocytes.

We have prepared I-labeled cholera toxin B subunit (I-labeled CT-B, a specific activity of 98Ci/mmol) and found that its binding to T and B lymphocytes from the blood of healthy donors was high-affinity (K 2.8 and 3.0nM, respectively).

Binding of Synthetic LKEKK Peptide to Human T-Lymphocytes.

The synthetic peptide LKEKK corresponding to sequence 16-20 of human thymosin-α1 and 131-135 of human interferon-α2 was labeled with tritium to specific activity 28 Ci/mol. The [3H]LKEKK bound with high affinity (Kd = 3.7 ± 0.