[MANIFESTATIONS OF EPIDEMIC PROCESS AND TRANSMISSION ROUTES OF CAUSATIVE AGENT OF ENTEROVIRUS SEROUS MENINGITIS].

Aim: Study the manifestations of epidemic process and leading transmission routes of causative agents of enterovirus serous meningitis (SM) by results of laboratory studies and epidemiologic examination of epidemic nidi.

Materials And Methods: During 2010 - 2014 a study for enterovirus was carried out in cerebrospinal fluid in 743 patients, hospitalized into medical organizations of Perm with primary diagnosis "serous meningitis", feces of 426 individuals, that had communicated with patients with SM of enterovirus etiology; 827 water samples from the distribution network, 295 water samples from open water and 57 washes from surface of vegetables and fruits. All the samples were studied in polymerase chain reaction, part--by a virological method.

[The frequency of detection of non-polio enteroviruses in foul and fecal waste waters, water and some food products].

In the Perm Territory from 2010 to 2014 155 samples offoul andfecal waste waters, 293 samples of surface water, 827 samples of supply net water, and 57 vegetable and fruit water-washes were examined for the RNA enterovirus agent with the use of polymerase chain reaction (PCR) method. In parallel 155 wastewater samples, 20 samples of surface water, and 4 samples of supply net water were examined for non-polio enterovirus agent with the use of virological methods. In the samples of foul waste waters the RNA enterovirus agent was detected in 74.

[The experimental evaluation of the possibility of the penetration of enteric viruses from the surface into the pulp of contaminated fruits and vegetables].

According to the results of complex microbiological examination of samples of vegetables, fruits and grapes there was established significant contamination of them with opportunistic bacteria, antigens of intestinal viruses and cysts of intestinal Protozoa, that confirms the epidemiological role of these products as factors in transmission of acute intestinal infections. There was revealed ribonucleic acid of enteric viruses in experimentally infected pulp from the surface of tomatoes and apples, that indicates to the possibility of penetration of these pathogens into the fruits and vegetables through intact (having no visible damages) surface.

[Influence of dairy food rotavirus contamination on morbidity].

The indirect hemagglutation test (IHT) indicated that rotaantigen infection of dairy foods (DF) selected at the milk processing shop in the year of high morbidity due to rotavirus infection (RVI) averaged 12.4%. Laboratory monitoring of DF sold in the year of relative low morbidity could establish that the rate of their rotaantigen contamination averaged 4.

[Evaluation of intestinal viral contamination of water objects as compared to morbidity trends].

The 1988-2000 laboratory studies of water objects in the Perm Region revealed a significant contamination of household effluents, underground and superficial water sources and distribution network water with hepatitis A antigen, Coxsackie and ECHO enteroviruses, and rotavirus antigen. A decrease in the contamination of distribution network water with hepatitis A antigen and an increase in its contamination with rotavirus antigen in recent years coincide with the trends of epidemic processes of respective infections. The maximum deterioration of the quality of distribution network water due to its contamination with hepatitis A antigen and rotavirus antigen is observed during spring freshet and precedes a seasonal rise in the incidence of hepatitis A and rotavirus infection in the population.