[Mutations in Escherichia coli pmp and htpR genes stabilize the products of foreign gene expression].

The half lives of mRNA for Escherichia coli chloramphenicol-acetyltransferase, Bacillus amyloliquefaciens alpha-amylase and human leucocyte interferon were measured in E. coli cells by molecular RNA.DNA hybridization.

[Stability of normal, abnormal and recombinant proteins in Escherichia coli strains deficient for intracellular proteinase La--the product of the lon gene].

Escherichia coli Lon-mutants deficient in intracellular protease La have been isolated. The rate of degradation of normal cellular proteins was 1.5-2-fold lower in Lon-mutants as compared with that of the wild type strain.

[Transcription of ribosomal protein genes rplKAJL and RNA-polymerase genes rpoBC in Escherichia coli cells: metabolic regulation of attenuation and the effect of rifampicin].

The E. coli genes rplKAJL specifying ribosomal proteins L11, L1, L10, L7/L12 are co-transcribed with the genes rpoBC encoding the beta- and beta'-subunits of RNA polymerase, but are separated by the site of attenuation. The efficiency of attenuation within rplKAJL-rpoBC operon was determined as a ratio of rplKAJL transcription frequency to the same of rpoBC genes.

[The role of ppGpp in the coordination of transcription of the ribosomal protein genes rplKAJL and RNA-polymerase rpoBC genes in Escherichia coli cells].

Transcription of the ribosomal protein genes rplKAJL and of the RNA polymerase genes proBC in the E. coli cells depends on the level of regulatory nucleotide ppGpp. The ppGpp acts as a negative regulator of transcription of the rpoBC genes in conditions of moderate deficiency of amino acids (after the cells were shifted down from amino acid rich to minimal media) or after incomplete deacylation of tRNA exerted by addition of serine-hydroxamate, or by partial inactivation of valyl-tRNA synthetase.

[Stringent control of relA gene transcription in cells of Escherichia coli].

Regulation of E. coli K12 relA gene transcription was studied. The rate of relA-RNA synthesis was measured by RNA-DNA-hybridization technique using cloned fragment of relA gene.