[Modulation of voltage-gated sodium channels by recombinant interferon-alpha2b in the human neuroblastoma cell line IMR-32].

Clonal human neuroblastoma cells imr-32 are a suitable model system for studies of neuronal excitability modulation. The ability interferon-alpha 2b "laferon" to modulate the mechanisms of electrical activity was studied in whole-cell patch-clamped undifferentiated human neuroblastoma cells IMR-32. It was shown that 1 h incubation of IMR-32 cells at 37 degrees C in medium with laferon (600 U/ml) exerted changes in voltage-dependent properties of Na(+)-channels.

[Surface charge of the cytoplasmic side of the cell membrane of neuroblastoma in murine line C1300].

Inside-out vesicles were obtained from the cell membranes of murine neuroblastoma. The surface charge of vesicles was studied by the microelectrophoresis method. At neutral pH they had the electrophoretic mobility 2.

[Relation between the surface potential of mouse neuroblastoma clone C1300 cells and the phase of the cell cycle].

The surface charge of neuroblastoma cells in different phases of the cell cycle was studied by the microelectrophoresis method. The surface charge increased by 50% on the average after addition of colchicine to the culture medium, by 20-30% after addition of dibutyryl-cAMP or removal of the serum from the medium and decreased by 30% after addition of DMSO. These changes correlated well with variations of the protein content per cell.

[Surface charge of mouse neuroblastoma cells and changes during growth and morphologic differentiation of the cell population].

Microelectrophoresis method was used to study the surface charge of murine neuroblastoma cells (clone C1300-N18TG2). It was shown that the surface charge of these cells was determined mainly by anionic groups of the membrane which were distributed with density 0.2 e/nm3 in the layer covering its outer surface.