[The structure of carbohydrate chains of human IgG4-paraproteins differing in the ability to bind cell receptors].

Comparative oligosaccharide analysis by HPLC revealed structural differences in the carbohydrate chains of human IgG4 paraproteins, varying in ability to induce the rhesus monkey's passive skin anaphylaxis. An atypical IgG4 paraprotein, which is inactive in this reaction and also does not bind the IgG4-subclass specific monoclonal antibody IH2, has a much higher proportion of the carbohydrate chains lacking terminal galactose residues than two typical IgG4 paraproteins. This structural feature may be one of the reasons for the atypical IgG4 not to bind by the mast cell Fc gamma receptor.

[A simple method of isolating monoclonal immunoglobulin M, possessing rheumatoid activity].

A simple procedure for obtaining highly purified preparations of native monoclonal (Waldenström's disease) immunoglobulin M possessing a rheumatoid activity (IgM-RF) has been developed. The method is based on the use of affinity chromatography with a new readily available adsorbent (immunoglobulin G-porous glass) and 3 M LiCl in Tris-buffer pH 8.3-8.

[The determination in saliva of IgA antibodies to Shigella ribosomes for the diagnosis of dysentery].

The levels of antiribosomal antibodies to Shigella ribosomes in serum and saliva samples from 38 dysentery patients (15 S. sonnei cases and 23 S. flexneri cases), 14 patients with salmonellosis and 136 healthy adults were determined in ELISA with ribosomes from S.

Protective milk O antibodies induced in guinea pigs by parenteral Shigella ribosomal vaccine.

IgG and IgA O antibodies were studied in milk and sera of guinea pigs subcutaneously immunized at various stages of pregnancy with Shigella ribosomal vaccines (SRV) from Shigella sonnei and Shigella flexneri. Both vaccines induced O antibodies in milk, the level of IgA antibodies being significantly higher than that of IgG antibodies. The immune milk provided a clear-cut protection against experimental Shigella-induced keratoconjunctivitis.