Washing and trypsin treatment of in vitro derived bovine embryos exposed to bovine viral diarrhea virus.

Gametes, somatic cells and materials of animal origin in media are potential sources for introducing bovine viral diarrhea virus (BVDV) into systems for production of IVF bovine embryos. Further, the efficacy of washing and trypsin treatment for removal of BVDV from IVF embryos is questionable. Washing and trypsin treatments recommended by the International Embryo Transfer Society for in vivo-derived embryos were applied to in vitro-derived, virus-exposed, bovine embryos in this side-by-side comparison of treatments.

Evaluation of analgesia induced by epidural administration of medetomidine to cows.

Objective: To evaluate analgesic effects after epidural administration of medetomidine to cows, compared with effects of lidocaine hydrochloride and 0.9% NaCl solution.

Animals: 6 adult beef cows.