The Effect of Oral Care Product Ingredients on Oral Pathogenic Bacteria Transcriptomics Through RNA-Seq.

Various ingredients are utilized to inhibit the growth of harmful bacteria associated with cavities, gum disease, and bad breath. However, the precise mechanisms by which these ingredients affect the oral microbiome have not been fully understood at the molecular level. To elucidate the molecular mechanisms, a high-throughput bacterial transcriptomics study was conducted, and the gene expression profiles of six common oral bacteria, including two Gram-positive bacteria (, ) and four Gram-negative bacteria (, , , and ), were analyzed.

The effect of an antioxidant gel compared to chlorhexidine during the soft tissue healing process: An animal study.

Background: Prolonged inflammation and oxidative stress can impede healing. To enhance healing efficiency, many solutions have been employed. This is an in vivo study comparing chlorhexidine (CHX) to a commercial antioxidant gel (AO).

High-Resolution Characterization of Enamel Remineralization Using Time-of-Flight Secondary Ion Mass Spectrometry and Electron Microscopy.

Introduction: The aim of this in vitro study was to assess the suitability of high-resolution time-of-flight secondary ion mass spectrometry (ToF-SIMS) for visualizing cross-sectional changes in human enamel microstructure and chemical composition during treatment and remineralization cycling of artificially generated caries lesions underneath an artificial plaque.

Methods: Treatments consisted of exposure to twice daily toothpaste/water slurries prepared from 0, 1,100, and 5,000 μg/g fluoride (F) NaF/silica toothpastes. In addition, treatments with slurries prepared from 1,100 μg/g F SnF2/silica toothpastes were done using 44Ca in the remineralization solution to allow for differentiation of newly formed mineral and exploration of incorporated metal dopants using ToF-SIMS.

In vitro safety evaluation of a hydrogen peroxide whitening emulsion technology on human enamel and dentin.

Purpose: To assess effects of a novel hydrogen peroxide leave-on whitening emulsion on surface hardness, fracture susceptibility, surface erosion, and surface morphology of enamel and dentin.

Methods: Human enamel and root dentin sections embedded in resin were leveled and polished for uniformity. A cycling treatment simulating overuse conditions (60 hours over 10 days), coupled with incubation in pooled human saliva at 37°C and two daily toothpaste treatments were used to evaluate the safety of a 3% hydrogen peroxide whitening emulsion treatment (Crest Whitening Emulsions).

Application of non-inferiority testing in microhardness and its relationship to erosion: Relevance for hard tissue safety evaluations.

Purpose: To use non-inferiority statistical testing with simple microhardness measurements (SMH) as a prediction of potential erosive hard tissue damage of topical treatments on enamel.

Methods: Three independent experiments of a simple acid cycling demineralization (ACD) model were used to screen softening effects of various commercial beverages on dental enamel. The cycling model consists of six repeated exposures of enamel slabs with alternating treatments of artificial saliva over the course of 6 hours.