Publications by authors named "E T Mishler"
Anti-Müllerian hormone (AMH) is widely used in the clinic as a biomarker for ovarian reserve and to predict ovarian response to gonadotropin stimulation. Patients with higher AMH levels tend to yield more oocytes and have better outcomes from assisted reproductive technology (ART) procedures. The goal of this study is to determine if AMH can be used to predict the outcome of controlled ovarian stimulation in rhesus macaques, which are commonly used in biomedical research, to refine animal use while maximizing oocyte yield.
View Article and Find Full Text PDFThe Sperm Chromatin Structure Assay (SCSA) is a robust test with high repeatability and precision. It is a clinically accepted assay that defines risk for infertility in men by measuring the degree of DNA fragmentation (% DFI) in sperm. The objective of this study was to adapt and validate the SCSA for rhesus macaques (Macaca mulatta) and establish a range for % DFI in fertile males.
View Article and Find Full Text PDFObtaining quality oocytes is a prerequisite for ART-based studies. Here we describe a method for transabdominal ultrasound-guided (US) oocyte retrieval in rhesus macaques () and compare it to the standard surgical approach using laparoscopy (LAP). We analyzed oocyte yield from six continuous reproductive seasons (2017-2023) that included = 177 US-guided and = 136 laparoscopic oocyte retrievals.
View Article and Find Full Text PDFObjective: To determine whether discontinuation of delta-9-tetrahydrocannabinol (THC) use mitigates THC-associated changes in male reproductive health using a rhesus macaque model of daily THC edible consumption.
Design: Research animal study.
Setting: Research institute environment.
Article Synopsis
- The study aimed to understand the cardiovascular effects associated with the administration of polidocanol both intravenously and through transcervical methods in rats and baboons.
- Systematic monitoring revealed that a significant dose-dependent increase in refractory hypotension occurred particularly with pressurized transcervical administration of polidocanol foam in baboons, while intravenous polidocanol also led to severe hypotension in all test subjects.
- Antihistamine pretreatments did not mitigate the hypotensive effects in baboons, highlighting the need for further research on the safety profile of polidocanol in these contexts.