Results of single-session bilateral triple pelvic osteotomy with an eight-hole iliac bone plate in dogs: 95 cases (1996-1999).

Objective: To evaluate feasibility of single-session bilateral triple pelvic osteotomy with 8-hole iliac bone plates in dogs with bilateral hip dysplasia.

Design: Retrospective study.

Animals: 95 dogs with bilateral hip dysplasia.

Effect of ewe ovine lentivirus infection on ewe and lamb productivity.

We used a previously described sensitive and specific ovine lentivirus (OLV) recombinant transmembrane (rTM) protein enzyme-linked immunosorbent assay (ELISA) to detect anti-OLV antibodies and define OLV infection in breeding ewes from nine US Meat Animal Research Center (MARC) flocks. We estimated the production impacts of dam rTM ELISA seropositivity on ewe and lamb productivity in the birth-to-weaning interval using production data from 1466 breeding ewes (of which 1242 actually lambed) and their 2452 lambs born in spring 1992 using several multiple linear and logistic regression models. By adjusting for lamb weaning age, gender, type of birth and rearing, birth difficulty, dam age, and flock, the component of ewe or lamb productivity related to ewe OLV infection alone was isolated.

Risk factors for seroprevalence of ovine lentivirus in breeding ewe flocks in Nebraska, USA.

The prevalence of and risk factors for ovine lentivirus (OLV) infection in 1466 breeding ewes in nine US Meat Animal Research Center (MARC) flocks were determined using a recombinant transmembrane protein (PTM) enzyme-linked immunosorbent assay (ELISA) to detect serum anti-OLV antibodies and define infection. Based on multivariable logistic regression, confinement birth and rearing (odds ratio (OR) = 1.6), older weaning ages (OR = 1.

Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157.

Identification of the O157 antigen is an essential part of the detection of Escherichia coli O157:H7, which is recognized as a major etiologic agent of hemorrhagic colitis. However, polyclonal antibodies produced against E. coli O157:H7 lipopolysaccharide (LPS) may react with several other bacteria including Brucella abortus, Brucella melitensis, Yersinia enterocolitica O9, Escherichia hermannii, and Stenotrophomonas maltophilia.

Monoclonal antibodies for detection of the H7 antigen of Escherichia coli.

Two murine monoclonal antibodies (MAbs) (2B7 and 46E9-9) reactive with the H7 flagellar antigen of Escherichia coli were produced and characterized. A total of 217 E. coli strains (48 O157:H7, 4 O157:NM, 23 O157:non-H7, 22 H7:non-O157, and 120 non-O157:nonH7), 17 Salmonella serovars, and 29 other gram-negative bacteria were used to evaluate the reactivities of the two MAbs by indirect enzyme-linked immunosorbent assay (ELISA).