[Drug treatment of coronavirus disease COVID-19: evidence exists?].

The article provides a review of foreign literature for 2020 on existing methods of drug treatment of coronavirus disease COVID-19. To date, in the treatment of COVID-19 in different countries, a little more than 10 drugs are used. The largest number of studies on the testing of these drugs is carried out by scientists from China, the USA, and European countries.

Rapid method for measurement of acetylcholinesterase activity.

Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities.

Fluorescent method for evaluation of cholinesterase inhibitors.

The sensitivity of detection of anticholinesterase compounds (e.g. tacrine) by the biochemical method significantly increased when fluorogenic compound N-(4-(7-diethylamino-4-methylcoumarine-3-yl)phenyl)maleimide was used instead of Ellman's reagent.

Rapid method for evaluation of cholinesterase inhibitors.

The fluorescence intensity of reversible inhibitor ethidium bromide fluorophore complex with equine blood butyryl cholinesterase decreases in the presence of inhibitor (tacrine) not fluorescing in the visible spectrum. An express method for tacrine evaluation is developed.

Express method for detection of Amanita phalloides amanitine toxins.

Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.