A Fermentation State Marker Rule Design Task in Metabolic Engineering.

There are several ways in which mathematical modeling is used in fermentation control, but mechanistic mathematical genome-scale models of metabolism within the cell have not been applied or implemented so far. As part of the metabolic engineering task setting, we propose that metabolite fluxes and/or biomass growth rate be used to search for a fermentation steady state marker rule. During fermentation, the bioreactor control system can automatically detect the desired steady state using a logical marker rule.

OptEnvelope: A target point guided method for growth-coupled production using knockouts.

Finding the best knockout strategy for coupling biomass growth and production of a target metabolite using a mathematic model of metabolism is a challenge in biotechnology. In this research, a three-step method named OptEnvelope is presented based on finding minimal set of active reactions for a target point in the feasible solution space (envelope) using a mixed-integer linear programming formula. The method initially finds the reduced desirable solution space envelope in the product versus biomass plot by removing all inactive reactions.

Closing the loop in bioproduction: Spent microbial biomass as a resource within circular bioeconomy.

Successful transition to a circular bioeconomy relies on the availability and efficient use of organic feedstocks such as agricultural and food waste. Advances in industrial biotechnology provide novel tools to valorize these feedstocks differently. Less attention, however, has been directed towards assessment of the organic side-residues arising from industrial biotechnology, such as spent microbial biomass (SMB).

Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by from Glycerol, Glucose and Ethanol.

Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. , a marine heterotrophic dinoflagellate, is successfully used for the industrial production of DHA because it can accumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities.

Sustainable metabolic engineering for sustainability optimisation of industrial biotechnology.

Industrial biotechnology represents one of the most innovating and labour-productive industries with an estimated stable economic growth, thus giving space for improvement of the existing and setting up new value chains. In addition, biotechnology has clear environmental advantages over the chemical industry. Still, biotechnology's environmental contribution is sometimes valued with controversy and societal aspects are frequently ignored.