Country-specific key lifestyle factors and health outcomes for resource allocation in the general population: a network analysis across 29 countries.

Background: We aimed to identify the central lifestyle, the most impactful among lifestyle factor clusters; the central health outcome, the most impactful among health outcome clusters; and the bridge lifestyle, the most strongly connected to health outcome clusters, across 29 countries to optimise resource allocation for local holistic health improvements.

Methods: From July 2020 to August 2021, we surveyed 16 461 adults across 29 countries who self-reported changes in 18 lifestyle factors and 13 health outcomes due to the pandemic. Three networks were generated by network analysis for each country: lifestyle, health outcome, and bridge networks.

Correction: Exploring ethical practice in NGOS on mental health research in Malawi.

[This corrects the article DOI: 10.1371/journal.pgph.

High-throughput DNA engineering by mating bacteria.

To reduce the operational friction and scale DNA engineering, we report here an DNA assembly technology platform called SCRIVENER (equential onjugation and ecombination for n ivo longation of ucleotides with low rors). SCRIVENER combines bacterial conjugation, DNA cutting, and homologous recombination to seamlessly stitch blocks of DNA together by mating in large arrays or pools. This workflow is simpler, cheaper, and higher throughput than current DNA assembly approaches that require DNA to be moved in and out of cells at different procedural steps.