Cross reactivity among human enterovirus serotypes as revealed by microneutralization assay technique.

Paired or tripled serum specimens examined for cross reactions between poliomyelitis viruses and other non-polio enteroviruses were obtained from 356 patients (217 males and 139 females) with various clinical diagnoses (aseptic meningitis, meningoencephalitis, paretic neuro-infections, upper respiratory tract infections, bronchopneumonia) who were sampled in 1984 and 1985. The sera were examined by the microneutralization assay technique in flat-bottomed 96-well polyvinyl chloride plates against 100-300 TCD50 of these enterovirus serotypes: CA9, CB1-CB5, E1, E2, E4-E9, E17, E20, E24, E30, EV71 and polioviruses P1-P3. The tests were carried out on BGM or RD cells.