Elicitation with methyl jasmonate combined with cultivation in the Plantform™ temporary immersion bioreactor highly increases the accumulation of selected centellosides and phenolics in (L.) Urban shoot culture.

(L.) Urban is an important pharmacopoeial plant used not only in medicine but also in cosmetology. agitated shoot cultures were established to study the influence of ethephon, methyl jasmonate, l-phenylalanine (Eth 50 µM, MeJa 50 µM, L-Phe 2.

Enhanced accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures analyzed by UPLC-MS/MS.

Harpagide and its derivatives have valuable medicinal properties, such as anti-inflammatory, analgesic and potential antirheumatic effects. There is the demand for searching plant species containing these iridoids or developing biotechnological methods to obtain the compounds. The present study investigated the effects of methyl jasmonate (MeJa, 50 μM), ethephon (Eth, 50 μM) and L-phenylalanine (L-Phe, 2.

Enhanced production of the pharmaceutically important polyphenolic compounds in L. shoot cultures by precursor feeding strategy.

Agitated L. shoot cultures were established to analyse the content of selected pharmaceutically important flavonoids and phenolic acids. Two variants (selected from nine ones) of MS medium were prepared: A (BAP 1 mg/L; NAA 0.

Seasonal changes of flavonoid content in Melittis melissophyllum L. (Lamiaceae).

Melittis melissophyllum L., a medicinal plant currently used in the folk medicine, was analyzed for the content of flavonoid compounds. The plants were collected in two locations in Poland in May and September.

The influence of L-phenylalanine, methyl jasmonate and sucrose concentration on the accumulation of phenolic acids in Exacum affine Balf. f. ex Regel shoot culture.

Phenolic acids are an important group of plant secondary metabolites with various, valuable therapeutic properties. Apart from plants growing in the open air, tissue cultures can be an alternative source of the secondary metabolites. The yield of their accumulation in in vitro cultures can be increased by different methods, including culture medium supplementation with precursors, elicitors and changing the standard amounts of the medium components.