Publications by authors named "E Sanada"
FOXO3a is a transcription factor involved in cell growth inhibition and apoptosis. FOXO3a is localized in the cytoplasm in cancer cells, and its nuclear translocation by small molecules is expected to prevent cancer cell growth. In this study, we screened a fungal broth library in HeLa cells using fluorescently labeled FOXO3a and an AI-based imaging system.
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- β-TrCP is a specific protein involved in the ubiquitin-ligase complex that helps target proteins for degradation, which is important for regulating various cellular processes.
- Researchers developed a high-throughput screening system to find small molecules that can specifically inhibit β-TrCP, leading to the identification of several compounds that affect the ubiquitin-proteasome system.
- The hit compounds not only inhibited the degradation of specific proteins like IκBα but also revealed key molecular features like carboxyl and hydroxyl groups that interact with β-TrCP, contributing to potential therapeutic applications in regulating cellular signaling.
Article Synopsis
- * A screening of 5,600 microbial extracts led to the discovery of a broth from sp. RK19-A0402 that significantly inhibits c-Myc transcriptional activity and is structurally related to oligomycin A.
- * The identified compounds cause mitochondrial dysfunction, resulting in reactive oxygen species (ROS) production that activates GSK3α/β to phosphorylate c-Myc for degradation, suggesting a potential approach for developing anticancer agents.
Article Synopsis
- c-Myc is an important regulator of cell growth and its overexpression is linked to many cancers, making it a target for potential therapies.
- Researchers have faced challenges in developing specific inhibitors for c-Myc, so they instead focused on small molecules that affect its cofactors or repressors.
- A study identified antimycin A as a promising compound that enhances c-Myc degradation via ROS, linking its effectiveness to c-Myc levels in cancer cells and suggesting a new direction for cancer treatment.
Allantopyrone A is an α-pyrone metabolite that was originally isolated from the endophytic fungus Allantophomopsis lycopodina KS-97. We previously demonstrated that allantopyrone A exhibits anti-cancer, anti-inflammatory, and neuroprotective activities. In the present study, we showed that allantopyrone A up-regulated the protein expression of hypoxia-inducible factor (HIF)-1α in human fibrosarcoma HT-1080 cells.
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