Diversity of actinomycete and their metabolites isolated from Howz Soltan Lake, Iran.

Saline environments are largely unexplored sources of actinomycetes with the potential to produce biologically active secondary metabolites. A total of 34 actinomycete isolates from water, sediments and mostly rhizosphere (82%) were collected from different sites at Howz Soltan Lake in Iran. Based on phylogenetic analysis, the isolates belonged to the genera Streptomyces, Nocardia and Saccharomonospora.

Melittin Prevents Metastasis of Epidermal Growth Factor-Induced MDA-MB-231 Cells through The Inhibition of The SDF-1α/CXCR4 Signaling Pathway.

Objective: Melittin is one of the natural components of bee venom (Apis mellifera), and its anticancer and antimetastatic properties have been well established, but the underlying mechanism remains elusive. The MDA-MB-231 is a triplenegative cell line that is highly aggressive and invasive. Besides, many critical proteins are involved in tumor invasion and metastasis.

Impacts of stratigraphic heterogeneity and release pathway on the transport of bacterial cells in porous media.

In order to manage and control the pathogen release from waste streams of various municipal, industrial, and agricultural pollution sources, it is crucial to investigate the impact of release pathways of such contaminants on their fate and transport in groundwater, especially in respect to natural heterogeneities encountered in aquifers. In this laboratory scale study, we investigate the impacts of different release scenarios of Escherichia coli bacteria, including spatially distributed surface recharge and single-point deep injection, as well as mono-pulse and continuous injection on the transport of Escherichia coli within both single-layered and multilayer aquifers. The results demonstrate earlier arrival of bacteria breakthrough curve (BTC) than conservative solute within a single-layer system with textural and continuum scale heterogeneities, attributed to size exclusion mechanism and preferential flow paths.

Prediction of the pho regulon in Streptomyces clavuligerus DSM 738.

Negative control of clavulanic acid by phosphate in Streptomyces clavuligerus DSM 738 suggests that a pho regulon may exist in this bacterium. S. clavuligerus PhoP was expressed with a C-terminal His-tag in Escherichia coli and purified.

Production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2.

Fifty strains of moderately halophilic bacteria were isolated from various salty environments in Iran. A strain designated as SA-2 was shown to be the best producer of extracellular lipase and was selected for further studies. Biochemical and physiological characterization along with 16S rDNA sequence analysis placed SA-2 in the genus Salinivibrio.