Metabolism of phosphonium choline by rat-2 fibroblasts: effects of mitogenic stimulation studied using 31P NMR spectroscopy.

Phospholipid turnover increases with both mitogenic stimulation and oncogenic transformation (1-9). Recent 31P nuclear magnetic resonance (NMR) spectroscopy studies of human tumors, animal tumor models and cell systems have reported elevated phosphomonoesters with growth and oncogenic transformation, as well as changes in these levels associated with treatment (10). In order to gain insights into the mechanisms underlying these changes, we used a phosphonium analog of choline and 31P NMR spectroscopy to study choline metabolism in quiescent and mitogenically stimulated Rat-2 fibroblasts.