[Ultrastructural and X-ray spectral analysis of cells U-937 during apoptosis process induced by hypertony].

The results of this work concerning ultrastructural changes of U-937 cells in a state of apoptosis are largely in consistent with the same information available in the literature. However, we have got the original data on the ultrastructural changes of cell organelles and immune localization and distribution of proteasomes. It has been demonstrated that Golgi apparatus is located close to the plasma membrane in the case of apoptosis induced by incubating the cells in a hypertonic suchrose solution (200-400 mM).

[Investigation of the kinetics of insulin amyloid fibrils formation].

Investigation of the structure of ordered protein aggregates--amyloid fibrils, the influence of the native structure of the protein and the environment on the process of fibrillation is currently the subject of intensive research. The present work is devoted to the study of the kinetics of insulin amyloid fibrils formation at low pH values (which are produced at many stages of the isolation and purification of the protein) using a fluorescent probe thioflavin T (ThT). It has been shown that the increase of fluorescence intensity of ThT during the formation of amyloid fibrils is described by a sigmoidal curve, in which 3 areas can be distinguished: the lag phase, the growth and the plateau, which characterize the various stages of fibril formation.

[The role of chromatoid bodies and cytoskeleton for differentiation of rat spermatozoids].

The ultrastructural and immunocytochemical study of rat male germ cells on different developing stages has been made. The investigation of morphological changes of spermatogenic cells has demonstrated the presence of tight connections between chromatoid bodies (CBs) and other cell organelles, particularly with the nucleus and Golgi apparatus; has revealed the association of manchette noncentrosomal microtubules (MT) with spermatid perinuclear ring plasma membrane (PM) in the zone of the adhesion intercellular contact--zonula adhaerens (ZA). The comparison of the results obtained in this work with available literary data has given possibility to analyze expected pathways of noncentrosomal MT nucleation in the late spermatids.

[The effect of red pigment of Saccharomyces cerevisiae on insulin fibril formation in vitro].

The effect of the yeast red pigment, the result of polymerization of AIR, and of its low molecular weight derivative (presumably devoid of phosphoribosyl moiety) on the formation of amyloid fibrils in vitro was studied. Both the red pigment and its derivative, the result of acid hydrolysis of the original pigment, were shown to diminish the intensity of amyloid bound Thioflavine T fluorescence. Correlation between the decrease of the intensity of Thioflavine T fluorescence and the concentration of both forms of the red pigment was demonstrated.

[Oligomeric forms, functions and cellular localization of alpha-crystallin type protein from Acholeplasma laidlawii].

Alpha-Crystallin type heat shock protein (alpha-HSP) IbpA from Acholeplasma laidlawii was expressed in Escherichia coil and isolated from cell extract on Ni-sepharose column. Recombinant IbpA, like other alpha-HSPs, spontaneously formed oligomeres in vitro. High resolution electron microscopy revealed regular structures with 15 nm in diameter.