Impacts of hnRNP A1 Splicing Inhibition on the Brain Remyelination Proteome.

Oligodendrocytes, the myelinating cells in the central nervous system, are implicated in several neurological disorders marked by dysfunctional RNA-binding proteins (RBPs). The present study aimed at investigating the role of hnRNP A1 in the proteome of the corpus callosum, prefrontal cortex, and hippocampus of a murine cuprizone-induced demyelination model. Right after the cuprizone insult, we administered an hnRNP A1 splicing activity inhibitor and analyzed its impact on brain remyelination by nanoESI-LC-MS/MS label-free proteomic analysis to assess the biological processes affected in these brain regions.

Dental appointments in the primary health care setting for users with toothache: a longitudinal analysis.

This study aimed to estimate the morbidity associated with toothache treated in the primary health care (PHC) setting, stratified by the population size of Brazilian municipalities, from 2018 to 2022. This longitudinal ecological study was based on public national data from routine public dental service utilization records within the Health Information System for Primary Care. These data were employed to compute the rate of dental appointments for users with toothache (per 1,000 users) within each municipality throughout 15 four-month periods (P1-2018 to P3-2022), classified by population size.

MonitoraSB: an innovation for monitoring and strengthening oral health in primary health care in Brazil.

Objective: This study describes the methodology used in the development of MonitoraSB, an innovation in the field of evaluating and monitoring oral health services in primary health care (PHC) in Brazil, and it discusses its characteristics and possible uses.

Methods: MonitoraSB includes a matrix of indicators and digital tools, developed in collaboration with oral health team dentists and oral health managers. The indicators evaluate the provision and management of oral health services and were developed on the basis of the model for evaluating the effectiveness of oral health care.

Distinct acute stressors produce different intensity of anxiety-like behavior and differential glutamate release in zebrafish brain.

Anxiety disorder is one of the most well-characterized behavioral disorders in individuals subjected to acute or chronic stress. However, few studies have demonstrated how different types of stressors can modulate the neurochemical alterations involved in the generation of anxiety. In this study, we hypothesize that subjects exposed to different aversive stimuli (mechanical, chemical, and spatial restriction) present varied intensities of anxiety-like responses associated with distinct patterns of gamma-aminobutyric acid (GABA) and glutamate release in the brain.