Publications by authors named "E S Matyugina"
To design a safe cellular system for testing inhibitors targeting the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2, a genetic construct was engineered containing viral cDNA with two blocks of reporter genes while the genes encoding structural S, E, and M proteins were absent. The first reporter block, consisting of Renilla luciferase and green fluorescent protein (Rluc-GFP), was located upstream of the SARS-CoV-2 5'-UTR. Meanwhile, the second block represented by firefly luciferase and red fluorescent protein (Fluc-RFP) was positioned downstream of the transcription regulatory sequence (TRS-N).
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- 5'-Noraristeromycin, a mixture of two enantiomers, has strong cytotoxic effects on leukemia cells, with different effectiveness across cell lines (Jurkat, K562, THP-1).
- In toxicity studies on white mice, the lethal dose (LD) of 5'-noraristeromycin was found to be around 63.2 mg/kg, with notable doses causing varying levels of acute toxicity.
- Administering this substance leads to systemic damage to the internal organs and tissues of the mice, indicating significant health risks at certain dosage levels.
We have previously shown that 5-arylaminouracil derivatives can inhibit HIV-1, herpesviruses, mycobacteria, and other pathogens through various mechanisms. The purpose of this study was to evaluate the potential of 5-arylaminouracils and their derivatives against leukemia, neuroblastoma, and glial brain tumors. 5-Aminouracils with various substituents and their 5'-norcabocyclic and ribo derivatives were screened for cytotoxicity against two neuroblastoma cell lines (SH-SY5Y and IMR-32), K-562 lymphoblastic cells, HL-60 promyeoloblastic cells, and low-passage variants of well-differentiated glioblastoma multiforme (GBM5522 and GBM6138).
View Article and Find Full Text PDFThe mechanisms of action of pyrimidine nucleoside derivatives on model lipid membranes of various compositions were studied. A systematic analysis of the tested agents' effects on the membrane physicochemical properties was performed. Differential scanning microcalorimetry data indicated that the ability of nucleoside derivatives to disorder membrane lipids depended on the types of nucleoside bases and membrane-forming lipids.
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- Enzymatic transglycosylation of a specific fleximer base using recombinant purine nucleoside phosphorylase (PNP) resulted in the production of both expected and unexpected reaction products, including N1-pyrazole nucleosides and novel derivatives.
- The study revealed that certain minor products, like a 4-imino-pyridinium riboside and bis-ribose derivatives, were formed, while a particular expected product (4-imino-pyridinium deoxyriboside) was absent.
- Molecular modeling helped identify how the structure of the heterocyclic substrates interacts with the PNP active site, suggesting that the spatial arrangement may facilitate secondary glycosylation, leading to