[Comparison of biological activity of high molecular weight and low molecular weight forms of immunoreactive prolactin from human serum in cultured lymphoma NB2 cells].

In experiments with NB2 rat lymphoma cells culture sensitive to lactogenic hormones the mitogenic activity of high molecular weight (> OOK) immunoreactive prolactin, found in substantial quantities in serum of certain hyperprolactinemic women, as compared to the activity of serum low molecular weight (23K) form, was studied. It was established that the ratio of immunoreactive to biologically active prolactin content in serums in cases of low molecular weight form predominance is close to 1,0 whereas in case of predominant content of high molecular weight form it is substantially higher (1.5-2.

[Heterogeneity of immunoreactive prolactin in hyperprolactinemia of various origin].

Study of molecular heterogeneity of immunoreactive prolactin in patients with macro- and microprolactinomas and idiopathic hyperprolactinemia has shown heterogeneity of the total blood immunoreactive prolactin pool in all the examined patients. This pool included three basic forms with molecular masses about 23, 50, and over 100 kD (23K-, 50K-, 100K-prolactin), whose ratios essentially differed in individual patients. Physiologically the most active monomeric 23K form of prolactin predominated in the blood of patients with hyperprolactinemia due to hypophyseal micro- and macroadenomas, parallelled by manifest signs of galactorrhea and hypogonadism; the content of this form may reach 95% of the total immunoreactive hormone.

[Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid].

Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Physico-chemical, immunochemical and biological properties of the amnionic prolactin were studied as compared with those of hypophyseal hormone. Properties of the hormonal forms isolated from both these tissues were similar.