[GENETIC EVALUATION OF CONTROL VARIANTS OF STRAINS SALMONELLA TYPHIMURIUM, USED IN THE TEST SALMONELLA/MICROSOME (Ames test)].

There were analyzed data of controls with solvents (DMSO and distilled water) of experiments performed in the laboratory of genetic monitoring in 2004-2014 on Salmonella typhimurium TA 100, TA 98 and TA 97 strains in the versions without and in the presence of metabolic activation. For all strains there was no revealed effect of the either solvent or system of metabolic activation on the average number of colonies of revertants in control. Generalized parameters (historical control) of the average number of colonies of revertants per plate: TA 100--number of experi- ments--317, average--118; 95% intervals of 71-165; TA 98--number of experiments--389, mean--22; 95% intervals 9-36; TA 97--the number of experiments--217, average--142; 95% intervals of 94-191.

[Buccal micronucleus cytome assay in complex genetic-hygienic study of preschool children].

There are presented results of the cytome analysis of epithelium of buccal mucosa in 167 children aged of 5-7 years, permanently residing in the city of Magnitogorsk and visiting municipal kindergartens. Frequencies of the main indices of genotoxic and toxic effects were: cells with micronuclei - 0.29±0.

[The effect of treatment of chrysotile with chloride iron (III) on the effect of genomic instability in cultured human lymphocytes].

All forms of asbestos are carcinogenic to humans, that caused the prohibition of its use in Europe, America, etc. However, the unique physical and chemical properties of asbestos and many opportunities for its applications in various industries and human activities require the creation of new technologies to. Analysis of genomic instability in human lymphocytes in a large range of doses (micronucleus test with cytochalasin B showed a decline of core indicators of genome instability (frequency of dividing cells with cytogenetic damage, asymmetry in the distribution of the genetic material in mitosis, proliferative activity) incubation of cells with asbestos modified chloride iron, as compared to the initial sample.

[Instability and sensitivity of the genome of healthy children in Magnitogorsk].

Problem of the influence of factors of the industrial city on the hereditary apparatus of its residents has not been fully resolved, because of traditionally in such studies only the pollution of environment components is taken into account. However the existence of a set of contributing socialfactors that modify the genotoxic effects ofpollution, requires the creation of a new methodology for genetic and toxicological studies. For this purpose, in Magnitogorsk, where one of Russia's largest steel plants is located, we conducted a comprehensive survey, whose tasks included the analysis of the influence of the complex of exogenous and endogenous factors on the genome of children.

[To a question on the impact of nanoparticles of metals present in the aquatic environment, on bacteria and continuous cell lines HEp-2 and BGM].

Promising application of nanoparticles and nanomaterials is the creation of sanitary hygienic means of new generation used for disinfection of water and indoor surfaces of mass use, furniture, sanitary technical equipment by virtue of modifying traditional materials to bring them effective biocidal properties, and for the development of methods in vitro for assessment their toxicity In this paper the possibility of the use various forms of silver, copper and aluminum as disinfectant for bacterial test organisms in the aquatic environment and assess their toxicity on biological models of continuous culture of BGM cells (a stable line of African green monkey kidney cells) and HEp-2 (Human epithelial type 2 (HEp-2) cells, derived from a human laryngeal carcinoma) is considered.