[MAST2-like protein kinase from grape vine Vitis vinifera: cloning of catalytic domain cDNA].

The aim of our work is the identification of protein kinases phosphorylating microtubule proteins in plant cells. Using bioinformatic approach, we found genes of putative homologues of microtubule-associated mammalian protein kinase MAST2 in higher plant genomes. The gene of closest MAST2 homologue, putative protein, named GMLK (Grape MAST2-Like Kinase, A7NTE9_VITVI), was found in grape Vitis vinifera.

[Cooperation between picornaviral proteins for the overcoming of cellular protective mechanisms].

Picornaviruses can overcome cellular mechanisms of antiviral defense by inhibiting cellular RNA and protein synthesis, nuclear-cytoplasmic transport and secretory pathways. The participation of proteases 2A and 3C and membrane proteins 2B and 3A in these processes is described. Four steps of the viral inhibition of cellular gene expression and the reciprocal supplement between these steps are discussed.