Ovarian cancer (OC) develops asymptomatically and escapes diagnosis until advanced stages, the feature contributing to a higher mortality rate. New prospects of OC diagnosis and treatment have been opened in studies of the gene regulation mechanisms that involve long noncoding RNAs (lncRNAs) and identification of the lncRNA genes that are inhibited via methylation of the promoter region. A set of 122 samples of primary OC tumors was examined by methylation specific real-time PCR to assess the methylation level of the lncRNA genes PLUT, SNHG1, SNHG6, SNHG12, and TINCR.
View Article and Find Full Text PDFVet Parasitol Reg Stud Reports
November 2024
Haemosporidians are heteroxenous, cosmopolitan protozoa that infect birds, using hematophagous dipterans as vectors. The genus Plasmodium has a moderate species diversity and is known for great flexibility in alternating host species, mild to severe pathogenicity, and generally low prevalence. Plasmodium huffi was first described in 1950 and considered insufficiently characterized, however, in 2021, this species was re-described as infecting two different Brazilian toucans, Ramphastos toco and Pteroglossus aracari.
View Article and Find Full Text PDFOur work aimed to evaluate and differentiate the role of ten lncRNA genes (, , , , , , , , , and ) in the development and progression of epithelial ovarian cancer (EOC). A representative set of clinical samples was used: 140 primary tumors from patients without and with metastases and 59 peritoneal metastases. Using MS-qPCR, we demonstrated an increase in methylation levels of all ten lncRNA genes in tumors compared to normal tissues ( < 0.
View Article and Find Full Text PDF() is the key pathogen of the porcine respiratory disease complex (PRDC) and contributes to pleurisy in pigs. Due to its limited metabolism and laborious cultivation, molecular tools are useful for diagnosis. This study investigated the genetic diversity of in slaughter pigs with pneumonia and pleurisy, and it assessed co-infections by type A (PM), (APP), and swine influenza virus A (sIVA).
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