DNA-damaging effects of dental bleaching agents.

We studied DNA-damaging effects of dental bleaching systems containing hydrogen peroxide and/or carbamide peroxide by the "comet assay" (alkaline version). Dental bleaching systems in a hydrogen peroxide concentration range from 0.03 to 30 mM produced a genotoxic effect on isolated HeLa cells in vitro comparable with the effects of pharmacopoeial hydrogen peroxide or urea peroxide.

Umbilical cord blood mesenchymal stem cells.

We studied umbilical cord blood mesenchymal stem cells and compared mesenchymal stem cells derived from umbilical cord blood, adipose tissue, and skin. Umbilical cord blood mesenchymal stem cells were characterized morphologically, cytofluorometrically, and by their differentiation potential. Umbilical cord blood mesenchymal stem cells did not differ from cells isolated from adipose tissue and skin by the main parameters (by morphology, expression of surface markers, and differentiation potential).

Differentiation potential of mesenchymal stem cells of different origin.

We compared differentiation potential of mesenchymal stem cells originating from human bone marrow, fatty tissue, thymus, placenta, and skin. The cells were characterized by differentiation into adipocytes and osteoblasts. Mesenchymal stem cells from different sources exhibited different differentiation potential, manifesting by the rate of differentiation and percentage of differentiated cells.

Comparison of mesenchymal stem cells obtained from different human tissues.

We studied mesenchymal stem cells from human bone marrow, adipose tissue, skin, placenta, and thymus. Morphological study and cytofluorometrical analysis by the main marker genes (CD10, CD13, CD31, CD44, CD90, CD105) were carried out. Mesemchymal stem cells of the studied tissues during isolation and culturing were morphologically similar and did not differ by the expression of the main marker genes.