Progenitor white cells and serum inhibitors in patients with bone marrow aplasia.

In patients with various blood diseases, especially with bone marrow hypoplasia following parameters were determined: presence of antibodies against autologous bone marrow, number of granulocyte-progenitor cells in short term bone marrow cultures and stimulating or inhibiting factors in patient's serum influencing the proliferative activity of bone marrow colonies. The results as well as possible clinical aspects are discussed.

[On the production of anti-human-lymphocyte serum (ALS) by means of combined local and intravenous immunization in swine].

Lymphocytes of donors' peripheral blood were used for the study of pig antihuman ALS preparation by means of local and intravenous immunization. The results were compared with those obtained with exclusively local application of the same antigen. Antisera were tested from the aspect of activity (lymphoagglutination, lymphocytotoxicity, the rosette inhibition test) and for the presence of unwanted antibodies (haemagglutinins, thromboagglutinins, precipitins to serum proteins).

[Use of the immune tolerance induction in the production of an antilymphocyte serum (ALS) free of antibodies against serum proteins].

The possibility has been investigated of a direct gain of ALS free of undesirable antibodies against serum proteins by inducing immunologic tolerance in productive animals (pigs). Preliminary experiments made with tolerogenic amounts of 10 and 50 ml of sera and with immunization by the serum alone proved applicability of this method. Electrophoresis showed antibodies against 6 to 7 and 2 to 3 fractions in animals tolerated with 10 and 50 ml respectively, compared to 18 to 20 fractions in the control group, which was not tolerated.

[Studies on the production of anti-human-lymphocyte serum (ALS) in bulls].

The applicability of bulls as productive animals was considered for the preparation of anti-humans ALS. The course of immunologic response was studied by lymphoagglutination, lymphocytotoxicity, rosette inhibition, hemagglutination tests and by precipitin formation in two experimental groups immunized by different amounts of lymphocytes from peripheral blood of normal donors. The animals were found to respond well already after the second application of very small amounts of antigen (on day 0-4 times 10(7), on day 21-2 times 10(8) lymphocytes).