Morphologic and gene expression analysis of periodontal ligament fibroblasts subjected to pressure.

Introduction: Force application (FA) during orthodontic tooth movement is mediated through periodontal ligament (PDL) fibroblasts. FA on deciduous teeth has an inherent risk of root resorption, which is less in permanent teeth. Currently, the root resorption mechanism is poorly understood.

Comparative proteomic analysis of human oral fluids according to gender and age.

Background: Recently, interest in finding disease bio-markers in human body fluids including oral fluids (OF), mainly saliva has increased. However, the physiologic differences in salivary proteins according to gender and age should be explored to establish a clinical diagnostic tool.

Objective: To compare OF protein expression according to gender and age, using proteomic approaches.

Different proteomic protein patterns in saliva of Sjögren's syndrome patients.

Objective: To investigate the salivary protein profile in patients with Sjögren's syndrome (SS), and healthy control subjects.

Materials And Methods: Unstimulated whole saliva samples were collected from 16 age-matched females; eight healthy subjects and eight patients diagnosed with SS (six primary SS, one incomplete SS and one primary SS associated with B cell lymphoma). Proteins were extracted and separated individually by 2D sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

Proteomic analysis of protein components in periodontal ligament fibroblasts.

Background: Characterization of periodontal ligament (PDL) fibroblast proteome is an important tool for understanding PDL physiology and regulation and for identifying disease-related protein markers. PDL fibroblast protein expression has been studied using immunological methods, although limited to previously identified proteins for which specific antibodies are available.

Methods: We applied proteomic analysis coupled with mass spectrometry and database knowledge to human PDL fibroblasts.