Redox dynamics in seeds of spp: unraveling adaptation strategies of different seed categories.

Background: Seeds of woody plant species, such as those in the like Norway maple ( L.) and sycamore ( L.), exhibit unique physiological traits and responses to environmental stress.

Modulating ascorbic acid levels to optimize somatic embryogenesis in (L.) H. Karst. Insights into oxidative stress and endogenous phytohormones regulation.

Global warming has adversely affected (L.) H. Karst.

Evaluation of P5CS and ProDH activity in (Steud.) as an indicator of oxidative changes induced by drought stress.

The aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (leaves and roots) was collected three times at 10-day intervals. The activity of enzymes involved in proline metabolism was closely related to drought severity; however, proline content was not directly impacted.

High-throughput method for Oxygen Consumption Rate measurement (OCR) in plant mitochondria.

Background: Conventional methods to measure oxygen consumption, such as Clark-type electrodes, have limitations such as requiring a large amount of starting material. Moreover, commercially available kits for high-throughput methods are usually optimized for animal cells and mitochondria. Here, we present a novel method to measure the oxygen consumption rate using a high-throughput assay in isolated mitochondria of European beech seeds.