[Typing of herpes simplex virus strains by analyzing the viral DNA using restriction endonucleases].

Analysis of the products of viral DNAs cleavage by restrictive endonucleases showed the strains of herpes simplex virus Us and L2 used for herpes vaccine manufacture to belong to type 1 and the VN strain to type 2 of herpes simplex virus. This method is found to be optimal for typing of herpes simplex virus strains.

[Experiment in infecting the green monkey Cercopithecus aethiops with a leukocyte virus isolated in hepatitis A].

The virus isolated in hepatitis A had been previously shown to passage in primary phytohemagglutinin-stimulated leukocyte cultures. The virus designated as leukocyte hepatitis virus (LHV) causes chromosome aberrations in the infected cultures. Some physico-chemical properties of LHV and its capacity for reproduction in other cell systems were studied.

[Etiological role of the herpes simplex virus in causing cervical cancer].

The results of the composite virological and immunological examinations of 46 patients with cervical carcinoma (CC) attest to the association of herpes simplex virus (HSV) with neoplastic processes. HSV strains were isolated from the tumour tissue as well as from the cervical canal secretions and blood of 4 patients. A higher level of virus-neutralizing and complement-fixing antibodies to HSV was found in sera from CC patients as compared with the control group of normal women.

[Further study of the leukocyte virus isolated in hepatitis A].

Two strains of leukocyte hepatitis virus (LHV) which had undergone 13 and 16 passages, respectively, in phytohemagglutinin-stimulated cultures of leukocytes from normal donors were studied in experiments with labelled precursors (3H-uridine and 14C-amino acids). The buoyant density of the virus (1,26 g/ml) and sedimentation constant of RNA (46 S) were determined. LHV was shown to be capable of reproduction in human kidney cell culture.