[Effect of salicylic acid on the proton translocation activity of plasmalemma of potato tuber cells].

Action of salicylic acid (SA) on the activity of membrane bound H(+)-ATPase and passive proton permeability of plasmalemma membrane vesicles (PMV) from parenchyma cells of potato tubers was detected. A correlation between SA action and germination of tubers and activity of plasmalemma H(+)-ATPase was revealed: the application of growth-stimulating concentrations of SA (10(-10)-10(-8) M) in the system in vitro resulted in activation of plasmalemma H(+)-ATPase, while the utilization of growth-inhibiting concentrations (10(-4), 10(-5) M) provoked inhibition of the enzyme activity. Addition of jasmonic acid (JA) to the incubation mix resulted in increase of SA effect on the accumulation of H+ in PMV.

[Melafen effect on ATP-dependent accumulation of calcium in plasma membrane vesicles from cells of potato tubers].

Synthetic growth regulator melafen (10(-5)-10(-10) M) was tested for an effect on the Ca2+ accumulation in plasma membrane vesicles (PMVs) isolated from potato Solanum tuberosum L. tubers at forced rest and sprouting. Melafen proved to regulate the Ca2+ accumulation in PMVs by changing the activity of Ca2+, Mg(2+)-ATPase of the plasma membrane, while no effect was observed with respect to Ca2+ outflow from vesicles.

[Interference of phytohormones and synthetic growth regulator melafen in the control of Ca2+ translocation across the plasma membrane of potato (Solanum tuberosum L) tuber cells].

Interference of phytohormones (jasmonic, gibberellic, and abscisic acids) and synthetic growth regulator melafen on Ca2+ translocation across the membrane of plasma membrane vesicles prepared from dormant potato (Solanum tuberosum L.) tubers was studied. The activity of plasma membrane Ca2+, Mg(2+)-ATPase was stimulated by melafen and jasmonic and gibberellic acids and suppressed by abscisic acid.

[Effect of jasmonic acid on Ca+2 transport through the plasmalemma of potato tuber cells].

The rate of Ca2+ accumulation in plasmalemma vesicles isolated from quiescent and sprouting potato (Solanum tuberosum L.) tubers and the effect of 10(-5) - 10(-10) M jasmonic acid on the accumulation of Ca+2 in plasmalemma vesicles and its efflux were studied. It was found that potato tuber plasmalemma contains a Ca+2, Mg+2-ATPase whose activity decreases upon the transition from forced quiescence to growth.

[Effect of melafen on the function of endoplasmic reticulum and plasmalemmal H+ -ATPase in the regulation of growth processes in potato tubers].

The mechanism of the stimulatory effect of melafen on potato tuber sprouting was studied. The treatment with 10(-8) M melafen intensified division and stretching and activated granular endoplasmic reticulum of apical meristem cells. An increase in the activity of membrane-bound H+-ATPase in the plasmalemma of parenchymal cells of melafen-treated potato tubers and enhancement of passive proton permeability of the plasmalemma was observed.