Publications by authors named "E Orellano"
Article Synopsis
- Bacterial pathogens can adapt to various environments, and understanding their transitions is vital for controlling diseases.
- In this study, Ralstonia solanacearum, responsible for bacterial wilt, was used to explore how it survives in water and soil, observing gene expression changes in these environments and in infected plants.
- Key findings indicate that stress responses and pathways for using alternative nutrients are crucial for survival in soil, while the type 3 secretion system is activated under specific conditions in water, highlighting important factors for the pathogen's life cycle.
- This research provides new insights into the biology and environmental adaptability of this plant pathogen.
Plant-pathogen interaction is influenced by multiple environmental factors, including temperature and light. Recent works have shown that light modulates not only the defense response of plants but also the pathogens virulence. Xanthomonas citri subsp.
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- Infestation with Varroa destructor parasites negatively impacts the survival of honey bee colonies (Apis mellifera), particularly in winter, making it crucial to assess infestation levels and apply treatments in autumn.
- The study highlights that re-invasion of treated colonies by Varroa mites can occur due to various factors, such as the status of untreated colonies and the layout of colonies within the apiary.
- Research conducted across six apiaries revealed that colonies arranged in a linear pattern and with more available brood are more susceptible to Varroa re-invasion, emphasizing the importance of strategic colony management for effective pest control.
The CRISPR/Cas9 system is widely used for editing genes in various organisms and is a very useful tool due to its versatility, simplicity, and efficiency. To teach its principles to post-graduate students we designed a laboratory activity to obtain and analyze PDS3 mutants in Arabidopsis thaliana plants consisting of: 1) Design of guide RNAs using bioinformatics tools; 2) plant transformation (which is optional depending on the length of the course); 3) observation and evaluation of the mutant's phenotypes in the Phytoene desaturase (PDS3) gene, which exhibit an albino phenotype and different degrees of mosaicism in the editing events we evaluated; 4) PCR amplification of a fragment that includes the mutated region followed by analysis of single-stranded DNA conformation polymorphisms (SSCP) using native polyacrylamide gel electrophoresis and silver nitrate staining to detect changes in the amplicon sequence due to gene editing. Through SSCP, the students were able to distinguish between homozygous and heterozygous edited plants.
View Article and Find Full Text PDFVarroa destructor is one of the most important sanitary threats for the beekeeping industry and so far disease control is based mainly on chemical treatment. However, a long-term solution may arise from studying natural surviving colonies of Apis mellifera. We compared the Varroa infestation rate in six commercial colonies that received annual treatment against mites and six non-treated colonies that survived in absence of any treatment for the last 6 years.
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