The expression analysis of the estrogen and progesterone receptors in endometrial carcinomas.

The aim of the study was the analysis of expression of estrogen (ER) and progesterone (PR) receptors in endometrial carcinomas. The expression of each of the receptors was examined with reference to such parameters as: patients' age, the relation to menopause, histological type and grading, the depth of myometrial infiltration and the presence of endometrial hyperplasia adjacent to the tumour. There were found very significant correlations between the degree of histological grading and expression of ER and PR and between values of index ER and PR.

[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer].

Objective: Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers.

Methods: Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots.

[Overall genomic DNA methylation in relation to estrogen].

Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors.