Computerized fluorescence microscopy of microbial cells.

The upgrading of fluorescence microscopy by the introduction of computer technologies has led to the creation of a new methodology, computerized fluorescence microscopy (CFM). CFM improves subjective visualization and combines it with objective quantitative analysis of the microscopic data. CFM has opened up two fundamentally new opportunities for studying microorganisms.

[Computerized optical image analysis as a tool for microbiological studies (review)].

Computerized optical image analysis is a variant of using computerized analysis of images of study objects recorded by optical methods. This review covers the literature and our own data on computer analysis of optical images of microbial origin. It is concluded that the use of this type of analysis in microbiology makes it possible to accelerate, objectify, and automate many conventional microbiological methods, as well as opens up new possibilities for studying single cells.

Single yeast cell vacuolar milieu viscosity assessment by fluorescence polarization microscopy with computer image analysis.

This study was undertaken to evaluate the apparent viscosity within the vacuoles of single Saccharomyces cerevisiae cells by steady-state fluorescence anisotropy measurements of quinacrine, using wide-field fluorescence polarization microscopy combined with computer image analysis. Quinacrine was shown to be rather specifically accumulated within the vacuoles of the cells. This accumulation was effectively reversed by ATP depletion of the cells, with no detectable binding of the dye within the vacuoles.

[Assessment of the distribution of nucleic acid intercalators in yeast cells by the pseudospectral image analysis].

The intracellular location of nucleic acid intercalators (NAI) in live (not fixed) Saccharomyces cerevisiae cells has been studied using fluorescence microscopy combined with computer pseudospectral image analysis. Three NAI: the anthracycline anticancer drug doxorubicin and the nucleic acid dyes ethidium bromide (E) and 4',6-diamidino-2-phenylindole (DAPI) were used. All three NAI were shown to be localized in nuclei and mitochondria.

Locating of nucleic acid intercalators in yeast cells by image analysis combined fluorescence microscopy.

Intracellular distribution in the intact (not fixed) Saccharomyces cerevisiae cells of the nucleic acid intercalators (NAI) was studied using fluorescence microscopy combined with computer image analysis (ImageJ software, NIH, USA). Three NAI-the anthracycline anticancer drug doxorubicin (DR) along with the nucleic acid dyes ethidium (E) and 4',6-diamidino-2-phenylindole (DAPI)-were used. Staining pattern and ImageJ quantitative analysis data provided evidence that all three NAI were located in the nuclei and in the mitochondria.