Gel-electrophoresis and subsequent optical emission (15)n analysis to identify (15)n-labelled protein fractions.

Abstract A combined procedure to detect of (15)N/(14)N isotope ratios by emission spectrometric analysis after starch gel-electrophoresis was developed. (15)N-labelled proteins of human serum were used to optimise this method. Electrophorised gel slices with protein fractions were directly digested for subsequent isotope analysis.